| 光谱学与光谱分析 |
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| Spectroscopic Research on the Interaction of 2-(4-Dimethylaminophenyl)-5-Fluoro-6-(Morpholin-4-yl)-1H-Benzimidazole and Calf Thymus DNA |
| TANG Ling-tian, WANG Yi, LIU Xin-qi，HU Shao-wen, CHU Tai-wei, WANG Xiang-yun* |
| College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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Abstract UV-Vis spectra of the aqueous solutions of 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl)-1H-benzimidazole (1) at different pH values revealed that compound 1 is a tertiary base, which can combine three protons. Through the non-linear least square method, the logarithm of the three accumulative protonation constants of compound 1, namely, lgβ1, lgβ2 and lgβ3, were determined to be 4.96±0.03, 5.72±0.07 and 7.95±0.10, respectively. UV-Vis and the steady-state fluorescence spectra indicated that a special interaction occurs between compound 1 and calf thymus DNA at the pH value of 3.40, of which the binding constant, Kb, is (2.30±0.10)×104 mol-1·L. Compound 1 in the concentration range of 10-8 to 1.2×10-6 mol·L-1 has a potential application to the quantitative determination of DNA.
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Received: 2004-02-26
Accepted: 2004-05-28
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Corresponding Authors:
WANG Xiang-yun
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| Cite this article: |
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TANG Ling-tian,WANG Yi,LIU Xin-qi, et al. Spectroscopic Research on the Interaction of 2-(4-Dimethylaminophenyl)-5-Fluoro-6-(Morpholin-4-yl)-1H-Benzimidazole and Calf Thymus DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1618-1621.
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https://www.gpxygpfx.com/EN/Y2005/V25/I10/1618 |
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