光谱学与光谱分析 |
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Study on Different Parts of Wild and Cultivated Gentiana Rigescens with Fourier Transform Infrared Spectroscopy |
SHEN Yun-xia1, 2, ZHAO Yan-li2, ZHANG Ji2, ZUO Zhi-tian2, WANG Yuan-zhong2*, ZHANG Qing-zhi1* |
1. College of Chinese Materia Medica, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China 2. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China |
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Abstract The application of traditional Chinese medicine (TCM) and their preparations have a long history. With the deepening of the research, the market demand is increasing. However, wild resources are so limited that it can not meet the needs of the market. The development of wild and cultivated samples and research on accumulation dynamics of chemical component are of great significance. In order to compare composition difference of different parts (root, stem, and leaf) of wild and cultivated G. rigescens, Fourier infrared spectroscopy (FTIR) and second derivative spectra were used to analyze and evaluate. The second derivative spectra of 60 samples and the rate of affinity (the match values) were measured automatically using the appropriate software (Omnic 8.0). The results showed that the various parts of wild and cultivated G. rigescens. were high similar the peaks at 1 732, 1 643, 1 613, 1 510, 1 417, 1 366, 1 322, 1 070 cm-1 were the characteristic peak of esters, terpenoids and saccharides, respectively. Moreover, the shape and peak intensity were more distinct in the second derivative spectrum of samples. In the second derivative spectrum range of 1 800~600 cm-1, the fingerprint characteristic peak of samples and gentiopicroside standards were 1 679, 1 613, 1 466, 1 272, 1 204, 1 103, 1 074, 985, 935 cm-1. The characteristic peak intensity of gentiopicroside of roots of wild and cultivated samples at 1 613 cm-1(C—C) was higher than stems and leaves which indicated the higher content of gentiopicroside in root than in stem and leaves. Stems of wild samples at 1 521, 1 462 and 1 452 cm-1 are the skeletal vibration peak of benzene ring of lignin, and the stem of cultivated sample have stronger peak than other samples which showed that rich lignin in stems. The iInfrared spectrum of samples were similar with the average spectral of root of wild samples, and significant difference was found for the correlation between second derivative spectrum of samples and average spectral of wild samples root, and the sequence of similarity was root>stem>leaf. Therefore, FTIR combined with second derivative spectra was an express and comprehensive approach to analyze and evaluate in the imperceptible differences among different parts of wild and cultivated of G. rigescens.
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Received: 2014-10-17
Accepted: 2015-02-20
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Corresponding Authors:
ZHANG Qing-zhi
E-mail: yzwang1981@126.com;ynkzqz@126.com
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[1] GUAN Ping, GAO Yu-qiong, SHI Jian-ming, et al(关 萍, 高玉琼, 石建明, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2005, 30(21): 1698. [2] MA Li-jie, YANG Bin, FENG Xue-feng, et al(马丽杰, 杨 滨, 冯学锋, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2010, 35(13): 1731. [3] YAN Yong-gang, GUO Xiao-heng, DENG-Chong(颜永刚, 郭小恒, 邓 翀). China Traditional and Herbal Drugs(中草药), 2011, 42(6): 1090. [4] ZHU Dong-hai, FU Mei-hong, YANG-Qing, et al(朱东海, 付梅红, 杨 庆, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2010, 35(13): 1758. [5] WANG Gang-li, YAO Ling-wen, ZHAI Wei-min, et al(王钢力, 姚令文, 翟为民, 等). China Traditional and Herbal Drugs(中草药), 2006, 37(7): 1093. [6] WU Hai, YI Lun-zhao, GAO Jing-ming, et al(吴 海, 易伦朝, 高敬铭, 等). China Traditional and Herbal Drugs(中草药), 2007, 38(9): 1298. [7] CHEN Sui-qing, LI Jun, CHEN Ying, et al(陈随清, 李 君, 陈 颖, 等). China Traditional and Herbal Drugs(中草药), 2009, 40(9): 1486. [8] ZHOU Tao, ZHANG Xiao-bo, GUO Lan-ping, et al(周 涛, 张小波, 郭兰萍, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2012, 37(13): 1917. [9] Ricardo J Lucio-Gutiérrez, Coello J, Maspoch S. Food Research International, 2011, 44: 557. [10] Adiana M A, Mazura M P. Journal of Molecular Structure, 2013, 1037: 40. [11] Li W L, Cheng Z W, Wang Y F, et al. Journal of Pharmaceutical and Biomedical Analysis, 2013, 72: 33. [12] Fan Q M, Chen C Y, Lin Y P, et al. Journal of Molecular Structure, 2013, 1051: 66. [13] Dimitrios Saltas, Christos S Pappas, Dimitra Daferea, et al. Journal of Agricultural and Food Chemistry, 2013, 61: 3235. [14] Choong Y K, Sun S Q, Zhou Q, et al. Journal of Molecular Structure, 2014, 1069: 60. [15] Li D, Jin Z X, Zhou Q, et al. Journal of Molecular Structure, 2010, 974: 68. |
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