光谱学与光谱分析 |
|
|
|
|
|
Preparation of Human Serum Albumin Micro/Nanotubes |
JIAO Pei-pei, GUO Yan-li*, NIU Ai-hua, KANG Xiao-feng* |
Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry & Materials Science, Northwest University, Xi’an 710127,China |
|
|
Abstract In this research, protein micro/nanotubes were fabricated by alternate layer-by-layer (LbL) assembly of human serum albumin (HSA) and polyethyleneimine (PEI) into polycarbonate (PC) membranes. The experimental conditions of pH values, ionic strength, the depositions cycles and the diameter of porous membrane were discussed. The morphology and composition of tubes were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), fourier transform infrared spectroscopy (FTIR) and energy dispersive spectroscopy (EDS). The results show that pH and ionic strength of the solution are the key factors that influence the effect of assembly. Micro/nanotubes with good opening hollow tubular structure were obtained when pH 7.4 HSA solution and pH 10.3 PEI solution without NaCl were used in synthesis procedure. The outer diameter of tube was dependent on the PC template, thus the micro/nanotubes size was controlled by the wall thickness, which can be adjusted by the number of layers of the HSA and PEI deposited along the pore walls. To avoid the thin wall being damaged in dissolving the template and vacuum drying, the PEI/HSA bilayer number should not be less than 3. The polar solvent N,N-dimethylformamide (DMF) can dissolve PC template to release the micro/nanotubes.
|
Received: 2015-03-22
Accepted: 2015-07-16
|
|
Corresponding Authors:
GUO Yan-li, KANG Xiao-feng
E-mail: guoyl@nwu.edu.cn; kangxf@nwu.edu.cn
|
|
[1] Kam N W S, Dai H. Journal of the American Chemical Society, 2005, 127(16): 6021. [2] Lee S B, Mitchell D T, Trofin L, et al. Science, 2002, 296(5576): 2198. [3] Son S J, Reichel J, He B, et al. Journal of the American Chemical Society, 2005, 127(20): 7316. [4] Komatsu T, Qu X, Ihara H, et al. Journal of the American Chemical Society, 2011, 133(10): 3246. [5] Qu X, Komatsu T. ACS Nano, 2010, 4(1): 563. [6] Hou S, Wang J, Martin C R. Nano Letters., 2005, 5(2): 231. [7] Yu A, Liang Z, Caruso F. Chemistry of Materials, 2005, 17(1): 171. [8] Perry J L, Martin C R, Stewart J D. Chemistry-A European Journal, 2011, 17(23): 6296. [9] Komatsu T. Nanoscale, 2012, 4(6): 1910. [10] Martin C R, Kohli P. Nature Reviews Drug Discovery, 2003, 2(1): 29. [11] Qu X, Lu G, Tsuchida E, et al. Chem. Eur. J., 2008, 14(33): 10303. [12] Carrillo J Y, Dobrynin A V. Langmuir, 2011, 28(2): 1531. [13] DeRocher J P, Pan Mao, et al. Macromolecules, 2010, 43(5): 2430. [14] Curry S, Mandelkow H, Brick P, et al. Nature Structural & Molecular Biology, 1998, 5(9): 827. [15] Sato T, Komatsu T, Nakagawa A, et al. Physical Review Letters, 2007, 98(20): 208101. [16] Kong J, Yu S. Acta Biochimica et Biophysica Sinica, 2007, 39(8): 549. |
[1] |
CHEN Shu-xin 1, 2, SUN Wei-min1*, SONG Yi-han3. Study on the Classification of LAMOST Early Stellar Spectrum Template by Mahalanobis Distance[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1618-1622. |
[2] |
CHEN Shu-xin1, 2,SUN Wei-min1*,KONG Xiao3. Similarity Measurement Among Classification Templates for LAMOST Stellar Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1922-1925. |
[3] |
YUAN Ting-ting, HUANG Peng, LI Han-yang, SHANG Yan-ting, YANG Xing-hua, ZHANG Yang, LI Han-yang, ZHAO En-ming*. Design and Research for a Kind of Optical Fiber Sensor Based on Extroradinary Optical Tramsimission Phenomenon[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 681-684. |
[4] |
JIANG Bin, LIU Shu-hui, WANG Wen-yu, GAO Jun*. Research on Automatic Parameter Measurement of WDMS Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(09): 2914-2918. |
[5] |
ZHANG Zheng-an1, 2, ZHENG Huai-li1, 3*, HUANG Fei2, FU Mao-mei4, LI Xiang1, 3, ZHOU Yu-hao1, 3. Ultraviolet-Initiated Template Polymerization of Cationic Polyacrylamide and Its Sludge Dewatering Performance[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(08): 2480-2485. |
[6] |
YU Jing-jing1, PANG Jing-chang1*, MENG Fan-long1, WEI Peng2. The Automatic Detection of the Continuum Problem in the Stellar Spectra Based on Distance Metric[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2246-2249. |
[7] |
PAN Xiao-hui, ZHANG Qin*, GUO Wei, CHEN Fa-he* . The SERS Detection of SudanⅠby Using AAO as Template to Prepare the Substrate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(06): 1556-1561. |
[8] |
FU Dong-mei, YU Xiao, TONG He-jun . Extracting Target from Blurred Midwave Infrared Image Based on Immune Template Clustering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(03): 673-676. |
[9] |
XU Jun1, XIE Cheng-wang2, LIU Hai-wen1, LIU Qiang3, LI Bin-cheng4 . The Improved Design of Encoding Mask and the Correcting Method for Recovered Spectral Images in Hadamard Transform Spectral Imager Based on DMD [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(05): 1419-1423. |
[10] |
GAO Wei-wei1, SHEN Jian-xin1, WANG Yu-liang1, LIANG Chun1, ZUO Jing2 . Algorithm of Locally Adaptive Region Growing Based on Multi-Template Matching Applied to Automated Detection of Hemorrhages [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(02): 448-453. |
[11] |
LIU Jie1, PAN Jing-chang1*, LIU Meng1, WEI Peng2, LUO A-li2 . Comparison of Different Template Matching Methods of Automatic Stellar Spectrum Parameter Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(02): 558-561. |
[12] |
LI Da-chao, YANG Jia, WU Peng, ZHU Rui, WANG Bo, LIN Yuan, XU Ke-xin . Effect of Borate Polymer Layers on Measurement of Glucose Concentration by SPR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(01): 142-146. |
[13] |
LIU Jie1, PAN Jing-chang1*, WEI Peng2, LIU Meng1, LUO A-li2 . Automatic Stellar Spectrum Parameter Measurement Based on Similarity Measure [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(12): 3402-3405. |
[14] |
LIU Ying1,2, FENG Jin-chao1, LI Jing2 . Preparation by Template Method and Spectral Characterization of α-MoO3 Nanorods and Nanometer Rectangular Pieces [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(02): 496-499. |
[15] |
LI Jun-mei1, XU Xiao-xuan1,2*, WANG Yu-fang1, WANG Bin1,2, SUN Jia-ming1,2,ZHANG Cun-zhou1,2. Using PAA Template to Fabricate SERS Substrate and the SERS Detection of Melamine [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(10): 2663-2666. |
|
|
|
|