光谱学与光谱分析 |
|
|
|
|
|
The Study on the Inueraction of FCLA with Human Serum Albumin with Spectroscopy Techniques |
ZHOU Jing,WEI Yan-chun* |
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China |
|
|
Abstract It is very important to detect reactive oxygen species (ROS) in biologic and medicinal research. FCLA, the Fluoresceinyl Cypridina Luciferin Analog, is a high sensitivity probe of detecting singlet oxygen and superoxide anions. The FCLA molecule can react with ROS and, after a series of reactions that result in the release of additional chemical energy, releases photons. Depending on the probe used, the photons are typically in visible wavelength range and easily measurable with conventional optical detectors. The binding reaction of FCLA with human serum albumin (HSA) was studied with spectroscopy techniques. It was found that HSA fluorescence was partially quenched by FCLA. The binding constants were obtained with quenching results. According to the Fōrster energy transfer mechanism, the energy efficiency and the distance between FCLA and HSA were calculated. Then it was found that static quenching was the main binding reaction between FCLA and HSA, and the energy transfer caused the HSA fluorescence quenching and the FCLA fluorescence producing. By studying the binding reaction of FCLA with HSA by spectroscopy techniques, this research proved the transfer style of FCLA and the interaction mechanism in the body. The results will offer some good advises about the ROS detection with the chemiluminesence probe in vivo. Meanwhile, it will also provide a new means to study pharmacokinetics and some basic theories about the medicine transfer in the body.
|
Received: 2006-08-28
Accepted: 2006-11-06
|
|
Corresponding Authors:
WEI Yan-chun
E-mail: weiyanchun@scnu.edu.cn
|
|
Cite this article: |
ZHOU Jing,WEI Yan-chun. The Study on the Inueraction of FCLA with Human Serum Albumin with Spectroscopy Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2287-2290.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2007/V27/I11/2287 |
[1] Harman D, Miquel J. Free Radical Theory of Aging. New York: Alan R Liss Inc, 1986. 3. [2] Akutsu K, Nakajima H, Katoh T, Kino S, et al. J. Chem. Soc. Perkin Trans.,1995, 2:1699. [3] WANG Juan,XING Da(王 涓,邢 达). Acta Biochem. Biophys. Sinica(生物化学与生物物理学报), 2002, 34(1): 11. [4] Zhu D B, Xing D, Wei Y D, et al. Luminescence, 2004, 19(5): 278. [5] He Y, Xing D, Yan G, et al. Cancer Lett., 2002, 182: 141. [6] Sibata C, Colussi V, Oleinick N, et al. J. Med. Biol. Res., 2000, 33(8): 869. [7] Salet C, Moreno G, Ricchelli F, et al. J. Biol. Chem., 1997, 272(35): 21938. [8] Qin Y F, Xing D, Luo S M, et al. Photochem. Photobiol., 2005, 81: 1534. [9] Wang J, Xing D, He Y,et al. The Federation of European Biochemical Societies Lett., 2002, 523: 128. [10] HE Yong-hong, XING Da,WANG Juan(何永红, 邢 达, 王 涓). Chinese Sci. Bull.(科学通报), 2003, 48(8):777. [11] He Y H, Xing D, Tang Y H, et al. Phys. Med. Biol., 2002, 47(9), 1535. [12] LIAN Shao-hui, YANG Shi-zhen, JIANG Shou-ping(连少辉, 杨士珍, 江寿平). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1994, 14(1): 33. [13] WU Yun-xia,XING Da(吴云霞, 邢 达). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(10):1630. [14] ZHANG Yong, YAN Xin-liang, LEI Ya-chun, et al(张 勇,燕新梁,雷亚春,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005,25(8):1274. [15] Takemura T, Ohta N, Nakajima S,et al. Photochem. Photobiol., 1992, 55: 137. [16] HAO Min, XING Da, CHEN Qun, et al(郝 敏,邢 达,陈 群,等). Chinese Chem. Lett.(中国化学快报), 2004, 15: 679. [17] Lakowicz J R. Principles of Fluorescence Spectroscopy(2nd Edition). New York:Kluwer Academic/Plenum Publishers, 1999. [18] Valensin G, Kushnir T,Navon G. J. Magn. Reson., 1982, 46: 23. [19] Eftink M R. Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. New York: Plenum Publishers, 1991. [20] Ware W R. J. Phys. Chem., 1962, 66: 455. [21] Jiang M, Xie M X, Zheng D, et al. J. Mol. Struct., 2004, 692: 71. [22] Kenworthy A K. Methods, 2001, 24: 289. [23] Saha D C, Ray K, Misra T N. Spectrochim. Acta, Part A, 2000, 56: 797. [24] ZHANG Xiao-wei, ZHAO Feng-lin, LI Ke-an(张小威, 赵凤林, 李克安). Chem. J. Chinese Universities(高等学校化学学报), 1999, 201(7): 1063. |
[1] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
[2] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
[3] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
[4] |
REN Li-lei, PENG Yu-ling, WANG Shu-jun*, ZHANG Cheng-gen, CHEN Yu, WANG Xin-tong, MENG Xiao-ning. Fluorescence Spectroscopy for Studies on the Interaction Between Three Metalloporphyrins With Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 806-813. |
[5] |
SHAO Ke-man, FU Gui-yu, CHEN Su-yan, HONG Cheng-yi, LIN Zheng-zhong*, HUANG Zhi-yong*. Preparation of Molecularly Imprinted Fluorescent Probe for Rare Earth Complex and Determination of Malachite Green Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 808-813. |
[6] |
HU Jing-jing, TONG Chang-lun*. Study on the Interaction Between Carbon Quantum Dots and Human Serum Albumin by Spectroscopic Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1107-1113. |
[7] |
ZHANG Jing1, GAO Xuan1, 2, JIN Liang1, WANG Hong-hui1, ZHOU Xi-ping1. Comparisons and Applications of Functional Equations for the Calculation of the Protein-Ligand Binding Constant Based on Fluorescence Spectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3494-3498. |
[8] |
JIA Hui-jie, ZHU Ning, GAO Yuan-yuan, WANG Ya-qi, SUO Quan-ling*. Effect of Substituent Structure of Benzothiazole Probe on Recognition to Metal Ion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3594-3598. |
[9] |
GUO Qing-ying1, LIU Min1,2*, ZHAO Yan-na2, WU Yu-shu2, SUN Bin2, LIU Jie1, HAN Jun2. Spectroscopic and Cytotoxicity Study on the Influence of (-)-Epigallocatechin-3-Gallate on the Interaction of Daunorubicin with Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1821-1827. |
[10] |
LIAN Jie1, REN Yi-fei2, YANG Rui-qin1*, HAO Hong-xia3. Rapid Detection System of 2,4,6-Trinitrophenol (TNP) Based on Fluorescent Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 804-808. |
[11] |
HAN Zhong-bao1, WU Yu-hang1, MI Yuan-yuan1, LIU Li-yan1,2, SU Gui-tian1, YU Zhan1,2*. Isomeric Discrimination of Oleanolic and Ursolic Acids by Human Serum Albumin: a Joint Study of Fluorescence Spectroscopy and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2190-2195. |
[12] |
XIONG Shi-peng, CHEN Jian-bo*. Characterization of the Interactions between Alpha Arbutin and Human Serum Albumin with Spectroscopic Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3489-3494. |
[13] |
WANG A-mei1, TU Zong-cai1, 2, WANG Hui1*, MA Da1. Research in Glycated Progress and Products of Human Serum Albumin by Infrared and CD Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3090-3095. |
[14] |
GONG Han-qing, CHEN Jian-bo*. The Interaction between 4-Ethyl-2-Methoxyphenol and Human Serum Albumin Studied by Spectroscopic and Molecular Docking Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1869-1873. |
[15] |
FANG Qing1, DONG Cheng-yu1, WANG Yu1, LIU Ying1,2*. The Interaction between Rolitetracycline and Human Serum Albumin Using Multi-Spectral Methods and Molecular Modeling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 990-996. |
|
|
|
|