| 光谱学与光谱分析 |
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| Study on the Interaction of Atrazine and ct-DNA by Fluorescence Probe Technique |
| MA Jing2, MENG Qing-xiang1, REN Li-ping1*, ZHANG Tao2, MU Hao-jie2 |
1. College of Animal Science and Technology/Beef Cattle Research Center, China Agricultural University, China State Key Laboratory of Animal Nutrition, Beijing 100193, China
2. College of Science, China Agricultural University, Beijing 100193, China |
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Abstract The interaction atrazine and ct-DNA was studied using fluorescence probe and UV absorption spectroscopy technique. Ethidium bromide (EB) was used as a probe. The effects of atrazine, phosphate, concentration of ions, and potassium iodide on the fluorescence of ct-DNA-EB system were investigated. The results showed that the fluorescence quenching process of atrazine to ct-DNA-EB system was proved to be the simultaneous dynamic and static quenching model. The phenomena of fluorescence quenching of ct-DNA-atrazine system by KI and hyperchromicity and the red shift of DNA UV-absorption caused by atrazine indicated the existence of intercalation between atrazine and ct-DNA. The experiments of phosphate and ionic strength showed that atrazine could combine with the phosphate group of ct-DNA by non-characteristic static force, and this action could be inhibited by high ionic strength.
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Received: 2007-03-26
Accepted: 2007-06-29
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Corresponding Authors:
REN Li-ping
E-mail: renlp@cau.edu.cn
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[1] ZHANG Li-jin, MIN Shun-geng, LI Guo-xue, et al(张立金，闵顺耕，李国学，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005，25（5）：739.
[2] HUANG Jin-xiang(黄金祥). Occupational Health and Emergency Rescue(职业卫生与应急救援), 1996, 14(2): 49.
[3] QI Shu-min(齐树民). Progress of Pharmacology(药理学进展), 1985, 12: 319.
[4] SHI Deng-rong, ZHANG Tao, REN Li-ping, et al(石登荣，张 涛，任丽萍，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006，26（3）：509.
[5] Filipov N M, Pinchuk L M, Boyd B L, et al. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2005, 86(2): 324.
[6] Hecker M, Park J W, Murphy M B, et al. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2005, 86(2): 273.
[7] Mark R. Applied and Environmental Microbiology，1995，61(1)：297.
[8] LIU Xian, GAO Guo-fen, YANG Li，et al（刘 仙，高国粉，杨 丽，等）. Chemical Journal of Chinese Universities（高等学校化学学报）, 2007，28（1）：103.
[9] HU Min，YANG Xin, JIN Wei-jun, et al(胡 敏，杨 欣，晋卫军，等). Acta Pharmaceutica Sinica(药学学报), 1999，34(8)：608.
[10] CAI Zhi-qiang, HAN Jin-duo, LI Liang, et al(蔡志强，韩金多，李 亮，等). Journal of Jiangsu Institute of Petrochemical Technology(江苏石油化工学院学报), 2002，14（3）: 25.
[11] Kumar C V, Asuncion E H. J. Chem. Soc. Chem. Commun., 1992, (6)∶470.
[12] Kumar C V, Turner R S, Asuncion E H. J. Photochem. Photobiol., A: Chem., 1993, 74：231.
[13] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智，郑朱梓，等). Analytical Application on Spectrofluorimetry(荧光分析法). Beijing: Science Press(北京：科学出版杜)，1990. 263.
[14] REN Li-ping, JIANG Shu-ren, RAO Zhen-hong, et al(任丽萍，江树人，饶震红, 等). Journal of Instrumental Analysis(分析测试学报)，2004，23（6）：58.
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