光谱学与光谱分析 |
|
|
|
|
|
Research on Application of Fourier Transform Infrared Spectrometry in the Diagnosis of Lymph Node Metastasis in Gastric Cancer |
BAI Yue-kui1, YU Li-wei1, ZHANG Le1, FU Jing2, LENG Hui2, YANG Xiao-jun3, MA Jun-qiang3, LI Xiao-juan3, LI Xiu-juan3, ZHU Qing4, ZHANG Yuan-fu4, LING Xiao-feng5, CAO Wen-lan6* |
1. Department of General Surgery, Beijing Haidian Hospital, Beijing 100080, China 2. Department of Pathology, Beijing Haidian Hospital, Beijing 100080, China 3. Yuncheng Central Hospital of Shanxi Province, Yuncheng 044000, China 4. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 5. Department of General Surgery,Peking University Third Hospital, Beijing 100191, China 6. Department of Oncology, Xiyuan Hospital, China Academy of Chinese Medicinal Sciences, Beijing 100091, China |
|
|
Abstract To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of lymph node metastasis in gastric cancer by Fourier transform infrared (FTIR) spectrometry. FTIR spectra of surgically removed fresh lymph nodes were measured by FTIR via probe of attenuated total reflection (ATR). For each spectrum, 13 bands were indentified and assigned between 3 000 and 1 000 cm-1. Peaks in the spectra were measured and relative intensity ratios were calculated and compared between the spectra of Metastatic lymph nodes (MLN) and Non-metastatic lymph nodes(NMLN). Standard statistic analysis was performed. 720 lymph nodes were measured in 38 gastric cancer patients. Results show that there were significant differences between the FTIR of 540 MLN and 180 NMLN. (1) For the band related to nucleic acid: The ratios of I1 240/I1 460(p=0.015) and I1 080/I1 460(p=0.034)increasedin MLN, which shows that the relative quantity of nucleic acid was more in MLN than that in NMLN. (2) For the bands related to protein: The ratios of I1 640/I1 460(p=0.001) and I1 546/I1 460(p=0.027) increased in MLN, which shows that the relative quantity of protein was more in MLN.(3) For the bands related to lipid: The ratio of I2 855/I1 460 and I1 740/I1 460 decreased in MLN FTIR spectrum, indicating the lower relative quantity of lipid in MLN. (4) For the bands related to carbohydrate: The ratio of I1 160/I1 460(p=0.023) decreased in MLN FTIR spectrum, indicating the lower relative quantity of carbohydrate in MLN. The results demonstrate that the FTIR spectroscopy technique maybe develop into a promising method for in situ and quick intraoperative differential diagnosis of lymph node metastasis in gastric cancer.
|
Received: 2014-04-04
Accepted: 2014-07-25
|
|
Corresponding Authors:
CAO Wen-lan
E-mail: cwl.xyyy@163.com
|
|
[1] Parkin D M, Bray F, Ferlay J, et al. CA Cancer J.Clin., 2002, 55(2): 74. [2] XU Yi-zhuang, XU Zhi, LING Xiao-feng, et al(徐怡庄,徐 智,凌晓锋,等). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报),2007, 43(4): 441. [3] Lewis P D, Lewis K E, Ghosal R, et al. BMC Cancer,2010, 23(10): 640. [4] Gazi E, Dwyer J, Gardner P. J. Pat. Hol., 2003, 201(1): 99. [5] Liu Y, Xu Y, Liu Y, et al. Br. J. Surg., 2011, 98(3): 380. [6] Li Q B, Sun X J, Xu Y Z, et al. Clin. Chem., 2005, 51(2): 346. [7] Yi W S, Cui D S, Li Z, et al. Spectrochim. Acta A Mol. Biomol. Spectrosc., 2013, 101(15): 127. [8] Wang X, Qi Z, Liu X, et al. Cancer Epidemiol., 2010, 34(4): 453. [9] Rorke L B. Cancer, 1997, 79(4): 665. [10] Kendall C, Isabelle M, Bazant-Hegemark F, et al. Analyst, 2009, 134(6):1029. |
[1] |
BAI Xue-bing1, 2, SONG Chang-ze1, ZHANG Qian-wei1, DAI Bin-xiu1, JIN Guo-jie1, 2, LIU Wen-zheng1, TAO Yong-sheng1, 2*. Rapid and Nndestructive Dagnosis Mthod for Posphate Dficiency in “Cabernet Sauvignon” Gape Laves by Vis/NIR Sectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3719-3725. |
[2] |
WANG Wen-song1, PEI Chen-xi2, YANG Bin1*, WANG Zhi-xin2, QIANG Ke-jie2, WANG Ying1. Flame Temperature and Emissivity Distribution Measurement MethodBased on Multispectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3644-3652. |
[3] |
YU Run-tian1, MA Man-man1, QIN Zhao2*, LIU Guan-nan1, ZHANG Rui1, LIU Dong1*. Study on Diagnostics of Nano Boron-Based Composite Metal Particles in Dispersion Combustion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3252-3259. |
[4] |
LIU Fei1, TAN Jia-jin1*, XIE Gu-ai2, SU Jun3, YE Jian-ren1. Early Diagnosis of Pine Wilt Disease Based on Hyperspectral Data and Needle Resistivity[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3280-3285. |
[5] |
LIANG Wan-jie1, FENG Hui2, JIANG Dong3, ZHANG Wen-yu1, 4, CAO Jing1, CAO Hong-xin1*. Early Recognition of Sclerotinia Stem Rot on Oilseed Rape by Hyperspectral Imaging Combined With Deep Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2220-2225. |
[6] |
FENG Ying-chao1, HUANG Yi-ming2*, LIU Jin-ping1, JIA Chen-peng2, CHEN Peng1, WU Shao-jie2*, REN Xu-kai3, YU Huan-wei3. On-Line Monitoring of Laser Wire Filling Welding Process Based on Emission Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1927-1935. |
[7] |
HU Bin1, 2, WANG Pei-fang1, 2*, ZHANG Nan-nan3, SHI Yue4, BAO Tian-li1, 2, JIN Qiu-tong1, 2. Effect of pH on Interaction Between Dissolved Organic Matter and Copper: Based on Spectral Features[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1628-1635. |
[8] |
WU Run-min1, XIE Fei1, SONG Xu-dong1*, BAI Yong-hui1, WANG Jiao-fei1, SU Wei-guang1, YU Guang-suo1, 2. The Mechanism of Hydrocarbon Flame Soot Formation in Spectral
Diagnosis: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 1-8. |
[9] |
LI Yi-chao1, 2, FU Jia1*, LÜ Bo1*, HUANG Yao1, QIAN Jin-ping1, LU Zheng-ping1, FU Sheng-yu1, LI Jian-kang1, WEI Yong-qing3, LIU Dong-mei4, XIAO Bing-jia1. A Photoelastic Modulator Based MSE Spectroscopic Diagnostic on EAST[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 111-115. |
[10] |
ZHANG Yan1, 2, 3,WU Hua-rui1, 2, 3,ZHU Hua-ji1, 2, 3*. Hyperspectral Latent Period Diagnosis of Tomato Gray Mold Based on TLBO-ELM Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2969-2975. |
[11] |
HUANG Shi-cheng1, HUANG Yi-ming1, 2*, YANG Li-jun1*, YUAN Jiong1, LIN Zhi-xiong1, ZHAO Xiao-yan1. Analysis of Inverted Y-Shaped Arc Photoelectricity Characteristic of
Flux-Cored Wire Pulsed TIG Additive Manufacturing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2579-2586. |
[12] |
LI Huan-tong1, 2, ZHU Zhi-rong1, 2, QIAO Jun-wei1, 2, LI Ning3, YAO Zheng3, HAN Wei1, 2. Molecular Representations of Jurassic-Aged Vitrinite-Rich and
Inertinite-Rich Coals in Northern Shannxi Province by
FTIR, XPS and 13C NMR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2624-2630. |
[13] |
SHEN Zhuo-wei, LI Rui, WANG Yang, XU Xiao-guang, XIAO Zhen*. The Proceedings of Raman Spectroscopy in Cervical Cancer in Recent Five Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2213-2217. |
[14] |
YANG Jin-chuan1, 2, AN Jing-long1, 2, LI Cong3, ZHU Wen-chao3*, HUANG Bang-dou4*, ZHANG Cheng4, 5, SHAO Tao4, 5. Study on Detecting Method of Toxic Agent Containing Phosphorus
(Simulation Agent) by Optical Emission Spectroscopy of
Atmospheric Pressure Low-Temperature Plasma[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1728-1734. |
[15] |
ZHAO Yue2, MA Feng-xiang2, WANG An-jing1*, LI Da-cheng1, SONG Yu-mei2, WU Jun1, CUI Fang-xiao1, LI Yang-yu1, CAO Zhi-cheng1. Research on Electric Breakdown Fault Diagnosis Model of Transformer Insulated Oil Based on Fluorescent Double-Color Ratio[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1134-1138. |
|
|
|
|