光谱学与光谱分析 |
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In Vitro Safety Evaluation Study of Angong Niuhuang Wan |
WANG Xin-mei, ZHANG Su, WANG Mei-bo, XIA Jing, LI Li-min, WANG Ke, JI Shen |
Shanghai Institute for Food and Drug Control, Shanghai 201210,China |
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Abstract To study a vitro extraction method to determine soluble mercury and mercury species in Angong Niuhuang Wan and investigate a vitro safety evaluation method, the best extraction scheme was determined after a great deal of conditioning experiments focusing on how the solvent,purification,duration of extraction and purification etc impact on mercury extraction. Soluble mercury was determined by inductively coupled plasma mass spectrometry and mercury species were determined by high performance liquid chromatography/inductively coupled plasma mass spectrometry. Result show that the best extraction method of soluble mercury and its species was ultrasonic extraction in artificial intestinal juice at 37 ℃ for 2 hours, letting it stand for 20 hours and taking the supernatant. The method was reproducible,which could simulate the human body environment to maximum extent. Methyl mercury, ethyl mercury and Hg2+ were not found in the extracts of Angong Niuhuang, although the content of soluble mercury was high. It was concluded that the method could be applied to the vitro extraction and determination of soluble mercury and mercury species in Chinese traditional medicines containing cinnabar. Methyl mercury, ethyl mercury and Hg2+ could be taken as the targets of in vitro safety evaluation of Angong Niuhuang.
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Received: 2013-09-10
Accepted: 2013-12-13
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Corresponding Authors:
WANG Xin-mei
E-mail: wangxinmeimei@sina.com
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[1] WANG Jin-hua, YE Zu-guang(王金华, 叶祖光). China Journal of Chinese Meteria Medica(中国中药杂志), 2004, 29(4):119. [2] WANG Jin-hua, YE Zu-guang, LIANG Ai-hua, et al(王金华, 叶祖光, 梁爱华,等). China Journal of Chinese Meteria Medica(中国中药杂志), 2003, 28(7): 639. [3] ZENG Ke-wu, WANG Qi, YANG Xiao-da, et al(曾克武,王 旗,杨晓达,等). China Journal of Chinese Meteria Medica(中国中药杂志), 2007, 32(3):231. [4] ZHANG Ying-na, LI Yang-hua, ZENG Li-ling, et al(张映娜, 李旸华,曾丽玲, 等). Chinses Journal of Health Laboratory(中国卫生检验杂志), 2010(04): 758. [5] ZHANG Li, CHEN Yao(张 莉, 陈 垚). Chinese Pharmaceutical Affairs(中国药事),2006,12: 742. [6] DING Jing-hua, WANG Na, JIANG Hong, et al(丁敬华, 王 娜,姜 泓,等). Chemical Research(化学研究), 2010,2: 80. [7] LI Jun, YUAN Zhuo-bin, HAN Shu-bo, et al(李 珺,袁倬斌,韩树波,等). Journal of Capital Medical University(首都医科大学学报),2006,27(5): 647.
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