光谱学与光谱分析 |
|
|
|
|
|
Cloud Point Extraction for Determination of Mercury in Chinese Herbal Medicine by Hydride Generation Atomic Fluorescence Spectrometry with Optimization Using Box-Behnken Design |
WANG Mei1, 2, LI Shan1, 2, ZHOU Jian-dong1, 2, XU Ying1, 2, LONG Jun-biao3, YANG Bing-yi1, 2* |
1. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China 2. Guangdong Key Laboratory of Molecular Epidemiology, Guangzhou 510310, China 3. Huangpu Center for Disease Control and Prevention, Guangzhou 510700, China |
|
|
Abstract Cloud point extraction (CPE) is proposed as a pre-concentration procedure for the determination of Hg in Chinese herbal medicine samples by hydride generation-atomic fluorescence spectrometry (HG-AFS). Hg2+ was reacted with dithizone to form hydrophobic chelate under the condition of pH. Using Triton X-114, as surfactant, chelate was quantitatively extracted into small volume of the surfactant-rich phase by heating the solution in a water bath for 15 min and centrifuging. Four variables including pH, dithizone concentration, Triton X-114 concentration and equilibrium temperature (T) showed the significant effect on extraction efficiency of total Hg evaluated by single-factor experiment, and Box-Behnken design and response surface methodology were adopted to further investigate the mutual interactions between these variables and to identify their optimal values that would generate maximum extraction efficiency. The results showed that the binomial was used to fit the response to experimental levels of each variable. ALL linear, quadratic terms of four variables, and interactions between pH and Trion X-114, pH and dithizone affected the response value(extraction efficiency) significantly at 5% level. The optimum extraction conditions were as follows: pH 5.1, Triton X-114 concentration of 1.16 g·L-1, dithizone concentration of 4.87 mol·L-1, and T 58.2 ℃, the predicted value of fluorescence was 4 528.74 under the optimum conditions, and the experimental value had only 2.1% difference with it. Under the conditions, fluorescence was linear to mercury concentration in the range of 1~5 μg·L-1. The limit of detection obtained was 0.012 47 μg·L-1 with the relative standard deviations (R. S. D.) for six replicate determinations of 1.30%. The proposed method was successfully applied to determination of Hg in morindae Radix, Andrographitis and dried tangerine samples with the recoveries of 95.0%~100.0%. Apparently Box - Behnken design combined with response surface analysis method was considered to be well used for optimization of the cloud point extraction.
|
Received: 2013-09-29
Accepted: 2013-12-21
|
|
Corresponding Authors:
YANG Bing-yi
E-mail: e-yby@163.com
|
|
[1] YANG Xiao-xi, HE Lin, HUANG Cheng-zhi(杨晓溪, 何 琳, 黄承志). Scientia Sinica Chimica(中国科学: 化学), 2013, 43(1): 87. [2] WANG Dong, GUAN Hong-feng, LIU Xiao-qiu(王 冬, 关宏峰, 刘晓秋). Journal of Shenyang Pharmaceutical University(沈阳药科大学学报), 2009, 26(2): 152. [3] DAI Bo, JIN Hong-yu, TIAN Jin-gai(戴 博, 金红宇, 田金改). Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2008, 28(6): 1014. [4] CHEN Jian-guo, JIN Xian-zhong , CHEN Shao-hong(陈建国, 金献忠, 陈少鸿). Physical and Chemical Testing-Chemical(理化检验-化学分册), 2011, 47(11): 1366. [5] Sato N, Mori M, Itabashi H. Talanta, 2013,117: 376. [6] Labrecque C, Whitty-Léveillé L, Larivière D. Analytical Chemistry, 2013, 85: 10549. [7] Shah A Q, Kazi T G, Baig J A. Food Chem. Toxicol., 2010, 48(1): 65. [8] Aranda P R, Gil R A, Moyano. S. Talanta, 2008, 75(1): 307. [9] Shoaee H, Roshdi M, Khanlarzadeh N. Spectrochim. Acta A Mol. Biomol. Spectrosc., 2012, 98: 70. [10] Ulusoy H I, Gürkan R, Ulusoy S. Talanta, 2012, 88: 516. [11] Niazi A, Momeni-Isfahani T, Ahmari Z. Journal of Hazardous Materials, 2009, 165(1-3): 1200. [12] Chen H, Chen J, Jin X. Journal of Hazardous Materials, 2009, 172: 1282. [13] Sood S, Jain K, Gowthamarajan K. Colloids and Surfaces B, Biointerfaces, 2013, 113: 330. [14] Pawar Y B, Purohit H, Valicherla G R. International Journal of Pharmaceutics, 2012, 436(1-2): 617. [15] YANG Shi-quan, ZHANG Xi-cheng, LIU Jun-feng(杨实权, 张喜成, 刘军锋). Microbiology China(微生物学报), 2010, 37(1): 91. |
[1] |
LIU Pan1, 2, DU Mi-fang1, LI Zhi-ya1, GAO Ling-qing1, 3, HAN Hua-yun4, ZHANG Xin-yao1, 3. Determination of Trace Tellurium Content in Steel by Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3103-3108. |
[2] |
YU Xin, ZHOU Wei*, XIE Dong-cai, XIAO Feng, LI Xin-yu. The Study of Digital Baseline Estimation in CVAFS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2392-2396. |
[3] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, YUE Yuan-bo2, HU Xue-qiang2, CHEN Yu2, LI Xiao-jia1, 2*. The Detection of Mercury in Solutions After Thermal Desorption-
Enrichment by Energy Dispersive X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1117-1121. |
[4] |
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827. |
[5] |
JULDEZ Nurlan1,2,3, SHEN Jian1,2,3, LENG Xiao-ting3, CHAI Yi-di1,2,3, WANG Shi-feng3, HU Yuan3, CUI Hao-yue3, WU Jing1,2,3*. Study on Measurement of Mercury Ion in Water by Thiamine-Fluorescence Excitation-Emission Matrix[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1846-1851. |
[6] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, HU Xue-qiang2, LIAO Xue-liang2, YUE Yuan-bo2, LI Xiao-jia1,2, CHEN Ji-wen3. The Rapid Detection of Trace Mercury in Soil With EDXRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 734-738. |
[7] |
CHEN Hai-jie1, 2, MA Na1, 2, BO Wei1, 2, ZHANG Ling-huo1, 2, BAI Jin-feng1,2, SUN Bin-bin1, 2, ZHANG Qin1, 2, YU Zhao-shui1, 2*. Research on the Valence State Analysis Method of Selenium in Soil and Stream Sediment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 871-874. |
[8] |
FAN Nai-yun, LIU Gui-shan*, ZHANG Jing-jing, ZHANG Chong, YUAN Rui-rui, BAN Jing-jing. Hyperspectral Model Optimization for Protein of Tan Mutton Based on Box-Behnken[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 918-923. |
[9] |
OU Li-juan, AN Xue-zhong, LUO Jian-xin, WANG Ling-yun, BO Heng, SUN Ai-ming, CHEN Lan-lan. High-Sensitive and Rapid Fluorescencet Detection of Hg2+ Based on Poly(adenine)-Templated Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 164-167. |
[10] |
CHEN Hai-jie1, 2, MA Na1, 2, BAI Jin-feng1, 2, CHEN Da-lei3, GU Xue1, 2, YU Zhao-shui1, 2, SUN Bin-bin1, 2, ZHANG Qin1, 2*. Study on Determination of Se in Geochemical Samples by External Supply H2-Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2896-2900. |
[11] |
LIN Hai-lan1, 2, ZHU Ri-long1*, YU Lei2, CHENG Yong-xia3, ZHU Rui-rui2, LIU Pei2, REN Zhan-hong3. Determination of Arsenic, Mercury, Selenium, Antimony and Bismuth in Soil and Sediments by Water Bath Digestion-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1528-1533. |
[12] |
YANG Lei, LIN Bin-bin, ZHENG Qi-wei, WU Shu-lan, ZHENG Bing-yun, ZHU Zhi-fei, HU Wen-ying. Its Photochemical Recognition to Hg2+ and Preparation of Nitrogen and Sulfur-Codoped Carbon Dots by Sulfanilamide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(11): 3388-3394. |
[13] |
LIANG Piao-piao1, ZHOU Shan-shan1, XING Yun-xin1, LIU Ying1, 2*. Quantification of Trace Elements in Hair Samples from 156 Women Living in the Low-Selenium Region of Inner Mongolia by ICP-AES and AFS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2217-2222. |
[14] |
ZHAO Ming-yue1, 2, 3, CHENG Jun-qi1, 2, YANG Bing-cheng3, WANG Zheng1, 2*. Highly Sensitive Determination of Selenium, Arsenic and Mercury in Seawater by Hydride Generation Coupled with Solution Cathode Glow Discharge Optical Emission Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1359-1365. |
[15] |
Lü Li, LI Yuan, JING Mei-jiao, MA Meng-dan, PENG Yue-han, QIN Shun-yi, LI Liu-an*. Determination of Selenium in Three Kinds of Eggs by Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 607-611. |
|
|
|
|