光谱学与光谱分析 |
|
|
|
|
|
A Fluoride-Sensor for Kink Structure in DNA Condensation Process |
LIU Yan-hui1, 2, ZHANG Jing1, CHEN Ying-bing1, LI Yu-pu3, HU Lin1* |
1. Soft Condensed Matter Lab, College of Science, Guizhou University, Guiyang 550025, China 2. State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 100190, China 3. College of Forestry, Guizhou University, Guiyang 550025, China |
|
|
Abstract Bloomfield has pointed out that the kink structure occurs for sharp bending during DNA condensation process, until now, which has not been proved by experiments. Using UV Spectrophotometer, the effects of fluoride and chlorine on the polyamine-DNA condensation system can be detected. Fluoride and chlorine both belong to the halogen family, but their effects on spermine-DNA condensation system are totally different. Fluoride ions make blue-shift and hyperchromicity appear in the spermine-DNA condensation system, but chlorine ions only make insignificant hyperchromicity happen in this system. Both fluoride ions and chlorine ions only make insignificant hyperchromicity happen in spermidine-DNA condensation system. Based on the distinguished character of fluoride, a fluoride-sensor for “kink” structure in DNA condensation was developed and the second kind of “kink” structure only appear in the spermine-DNA condensation system.
|
Received: 2013-04-02
Accepted: 2013-07-21
|
|
Corresponding Authors:
HU Lin
E-mail: ionazati@itp.ac.cn
|
|
[1] Arenzon J J, Stilck J F, Levin Y. Eur. Phys. J. B, 1999, 12: 79. [2] Manning G S. Biophysical Journal, 2006, 91: 3607. [3] Chih-chen Hsieh, Balducci A, Doyle P S. Nano Letters, 2008, 8: 1683. [4] Christine C Conwell, Igor D Vilfan, Nicholas V Hud, PNAS, 2003, 100: 9296. [5] Saito T, Iwaki T, Yoshikawa K. Biophysical Journal, 2009, 96: 1068. [6] ZHANG Jing,HU Lin,LIU Yan-hui(张 婧,胡 林,刘艳辉). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(5): 1306. [7] Patricia G Arscott, Li Anzhi, Victor A. Bloomfield, Biopolymers, 1990, 30: 619. [8] Eric G Plum, Patricia G Arscott, Victor A. Bloomfield, Biopolymers, 1990, 30: 631. [9] Greenwood N N, Earnshaw A. Chemistry of the Elements, 2n ed. Oxford, Bufferworth—Heinemann, 1997. [10] Filip Lankas, Richard Lavery, John H. Maddocks, Structure, 2006, 14: 1527. [11] Mitchell J S, Laughton C A, Sarah A Harris. Nucleic Acids Research, 2011, 39: 3928. [12] Troy A Lionberger, Davide Demurtas, Guillaume Witz,et al. Nucleic Acids Research, 2011, 39: 9820. [13] Chen H, Liu Y, Zhou Z, et al. Physical Review E, 2009, 79: 041962. [14] Amin Ahmed Ouameur, Heidar-Ali Tajmir-Riahi. Journal of Biological Chemistry, 2004, 279: 42041. [15] Burt G Feuerstein, Nagarajan Pattabiraman, Laurence J. Marton, Nucleic Acids Research, 1990, 18: 1271. |
[1] |
HUANG Xing-zhong1, 2, WU Wen-fen1, LI Zhan-bing1, LI Hui-quan1, 2, LIU Qing-qing1, LI Shao-peng1*. Spectral Analysis of Fluoride and Nitride Phase in Secondary Aluminum Dross[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3588-3594. |
[2] |
TENG Yuan-jie, WEI Qi-zhen, LIU Wen-han, LIU Jiang-mei, NIE Yong-hui, LI Pan. Cy3-Labeled Aptamer Combined with Surface-Enhanced Raman Scattering Using for Specific Detection of Trace Acetamiprid[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2462-2467. |
[3] |
WANG Zhi-hui1, LONG Dan-dan2, LI Zi-juan2, YANG Ya-fei3, YAN Jing-hui2*, ZOU Ming-qiang4,5*. One-Pot Microemulsion Synthesis of NaYF4∶Yb3+, Ho3+@Au[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1793-1797. |
[4] |
GONG Hong-ling1, LIU Yan-hui2*, TANG Yan-lin2*, HU Lin2. Effects of Temperature on DNA Condensation Detected with Temperature-Changed Ultraviolet Spectrum Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1831-1837. |
[5] |
ZHANG Jing1, HU Lin2*, LIU Yan-hui2, ZHANG Bi-cheng1 . Research on the DNA Condensation Properties by Ultraviolet Spectrum [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(05): 1306-1309. |
[6] |
HU Yue-bo1, 2, 3, ZHANG Xin-na2, ZHOU Da-li3, JIAO Qing1, WANG Rong-fei1, HUANG Jin-feng2, LONG Xiao-bo2, QIU Jian-bei1* . Effect of Bivalent Alkaline Earth Fluorides Introduction on Thermal Stability and Spectroscopic Properties of Er3+/Tm3+/Yb3+ Co-Doped Oxyfluorogermanate Glasses[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(01): 56-60. |
[7] |
ZHANG Xiang-hua,LIU Zheng-wei,XIAO Si-guo . Direct Sensitization Up-Conversion Mechanism from Yb3+ to Ho3+ and Tm3+ in Fluoride [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(05): 660-664. |
|
|
|
|