光谱学与光谱分析 |
|
|
|
|
|
The Detection of Flavonoids Glycosides from Ginkgo Biloba Based on Spectrofluorimetry |
SHAO Ju-fang, WEN Zong-zhuang, ZHU Hong-wei |
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, China |
|
|
Abstract Flavonoids glycosides from ginkgo are widely applied in pharmaceutical, food, daily necessities and other areas due to their variety of biological activities. To overcome the shortcoming of current methods of time wasting, complex operation and expensive costs, an accuracy, low costs and fast determination method was established for flavonoids glycosides from ginkgo. According to the principle that flavonoids can form fluorescent chelate with Al3+, the detection condition was explored with rutin as standard. The results showed that the fluorimetric intensy of chelate of rutin with Al3+ would be stabilized and achieve maximum with λex=400 nm and λem=520 nm, in Al(NO3)3-(HAc-NaAc) reaction system for 1 500 s with pH 3.6. The linear regression equation y=29.92x+36.49 (R2=0.986) was deduced with the concentration of rutin and fluorescence intensy, and the linear range 1.8×10-6~3.2×10-5 mol·L-1. Flavonoids glycosides of cell suspension cultures from ginkgo biloba was detected by this method. The recovery experiments were also carried out with the average recovery rate of 101.3%. The advantages of high sensitivity, reproducibility, simple operation, and low costs were showed, indicating its good prospects.
|
Received: 2013-05-03
Accepted: 2013-08-09
|
|
Corresponding Authors:
SHAO Ju-fang
E-mail: jfshao@cumt.edu.cn
|
|
[1] Shibata S. Stem. Call, 1994, 12(1): 44. [2] Zhu H, Wang Y, Liu Y, et al. Food Analytical Methods, 2010, 3 (2): 90. [3] Wang Y, Chu H T. Advanced Materials Research, 2011, 236: 2630. [4] Qing L S, Xue Y, Zhang J G, et al. Journal of Chromatography A, 2012, 1249 (6): 130. [5] Anja K, Markus G. Journal of Pharmaceutical and Biomedical Analysis, 2012, 70(5): 553. [6] Zhao X, Zhao Y L, Liu X M, et al. Journal of Seperation Science, 2012, 35(8): 984. [7] Chang C C, Lee S S. Chemistry and Natural Compounds, 2012, 48(4): 689. [8] Zhang Y Y, Li Z C, Zhu J K, et al. Chinese Chemical Letters, 2010, 21 (10): 1231. [9] Chi L, Li Z, Dong S, et al. Microchimica Acta, 2009, 167(3): 179. [10] Serbia V S, Stefana D. Acta Chim. Slov., 2009, 56: 967. [11] Chen X P, Zhang Y D. Journal of Chromatographic Science, 2008, 46(2): 117. |
[1] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[2] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
[3] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, YUE Yuan-bo2, HU Xue-qiang2, CHEN Yu2, LI Xiao-jia1, 2*. The Detection of Mercury in Solutions After Thermal Desorption-
Enrichment by Energy Dispersive X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1117-1121. |
[4] |
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827. |
[5] |
LIU Yu1, LI Zeng-wei2, DENG Zhi-peng1, ZHANG Qing-xian1*, ZOU Li-kou2*. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2817-2822. |
[6] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
[7] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, HU Xue-qiang2, LIAO Xue-liang2, YUE Yuan-bo2, LI Xiao-jia1,2, CHEN Ji-wen3. The Rapid Detection of Trace Mercury in Soil With EDXRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 734-738. |
[8] |
LIU Chong-hua, OUYANG Yu, CHEN Guan-qian, PENG Cai-hong, SONG Wu-yuan. Rapid Determination of Chromium, Arsenic, Selenium, Cadmium, Antimony, Barium, Mercury and Lead in Toy Plastics by Energy Dispersive X-Ray Fluorescence Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 739-744. |
[9] |
ZHAO Ting1,2,3, CHI Hai-tao1,2,3*, LIU Yi-ren1,2,3, GAO Xia1,2,3, HUANG Zhao1,2,3, ZHANG Mei1,2,3, LI Qin-mei1,2,3. Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 750-754. |
[10] |
ZHAO Hong-kun1, 2, YU Tian3, XIAO Zhi-bo3, HAO Ya-bo4, LIU Ya-xuan1*. Homogeneity Test of Geochemical Certified Reference Materials by X-Ray Fluorescence Spectrometry With Pressed-Powder Pellets[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 755-762. |
[11] |
CHEN Hai-jie1, 2, MA Na1, 2, BO Wei1, 2, ZHANG Ling-huo1, 2, BAI Jin-feng1,2, SUN Bin-bin1, 2, ZHANG Qin1, 2, YU Zhao-shui1, 2*. Research on the Valence State Analysis Method of Selenium in Soil and Stream Sediment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 871-874. |
[12] |
CHEN Hai-jie1, 2, MA Na1, 2, BAI Jin-feng1, 2, CHEN Da-lei3, GU Xue1, 2, YU Zhao-shui1, 2, SUN Bin-bin1, 2, ZHANG Qin1, 2*. Study on Determination of Se in Geochemical Samples by External Supply H2-Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2896-2900. |
[13] |
XIONG Yang1,2, XU Jun1,2, QIU Su-yan1,2, WEI Yi-hua1,2, ZHANG Jin-yan1,2*. Study on Interaction Between Phenylethanolamine A and CdTe Nanomaterials by Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1402-1406. |
[14] |
ZHAO Yu-yan, ZHANG Ze-yu, TANG Xiao-dan*, ZANG Li-bin, LIU Xu-yang, LU Ji-long. Analysis of High Content Water-Soluble Salt Cation in Saline-Alkali Soil by X-Ray Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1467-1472. |
[15] |
LIN Hai-lan1, 2, ZHU Ri-long1*, YU Lei2, CHENG Yong-xia3, ZHU Rui-rui2, LIU Pei2, REN Zhan-hong3. Determination of Arsenic, Mercury, Selenium, Antimony and Bismuth in Soil and Sediments by Water Bath Digestion-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1528-1533. |
|
|
|
|