光谱学与光谱分析 |
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Observation of the Pollen Mother Cell Meiosis of Male Sterile Line in Rice by Using Confocal Microscopy |
ZHANG Jia-jia, DAI Xi-mei*, ZHAO Shuai-peng, LIU He-mei, HUANG Qun-ce, JIAO Zhen |
Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University,Zhengzhou 450052, China |
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Abstract In the present paper, meiosis of male sterile pollen mother cells and fertility of ripened pollen was observed in male sterile line (Xindao97200A) by using laser scanning confocal microscopy (LSCM), beside, the seed setting rate of selfing and artificial pollination was investigated. Abnormal chromosome behaviors during the meiosis of PMC were found with 15.8%, which included lagging chromosome, dragging chromosomes, three poles of spindles, chromosome bridge, micronuclei and asynchrony of the dyad. Typical, spherical and stained abortive rates were 6.15%, 10.26% and 80.77%, respectively. The pollen abortion might be attributed to abnormal pollen development, which resulted from chromosome pairing abnormally, abnormal division, chromosome breakage and unevenly distributed cytoplasm.
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Received: 2013-01-04
Accepted: 2013-04-10
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Corresponding Authors:
DAI Xi-mei
E-mail: daiximei@yahoo.com.cn
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