光谱学与光谱分析 |
|
|
|
|
|
Noninvasive Medical Imaging System for Tissue Classification Using RGB LED and Micro-Spectroscopy |
YANG Bor-wen1, LIN Yu-min2, WANG Shih-yuan1, YING Shang-ping1 |
1. Department of Opto-Electronic System Engineering, College of Engineering, Minghsin University of Science and Technology, Hsinchu 30401, Taiwan, China 2. Institute of Electronic Engineering, College of Engineering, Minghsin University of Science and Technology, Hsinchu 30401, Taiwan, China |
|
|
Abstract As skin is the exterior organ of human body, cosmetic industry advances year by year. To reveal the details of skin tissue, three-dimensional medical imaging is required. Based on the idea of “readout instead of write”, a new scheme named spectral classification imaging (SCI) is proposed in the present study to reduce the invasiveness by applying the reflection spectra of the sample points for three-dimensional medical imaging. Broad-band light source and the spectrometer were employed to collect the spectra curves of scanned region, which were classified into several tissue types by their cross-correlations. A colorful tissue tomography can finally be obtained by filling in each image pixel the color indicating the corresponding tissue type. The lateral/longitudinal resolutions and penetration depth were analyzed to characterize the SCI system. The lateral resolution is based on the source’s diffraction limit, the longitudinal resolution is by its depth-of-focus, and the penetration depth is equivalent to its skin depth. The imaging results of an amethyst of 0.6 mm (x-direction)×0.6 mm (y-direction) with a total of 120×120 pixels per frame and a guppy fish of 3.2 mm (x-direction)×2.4 mm (y-direction) of 160×120 pixels, are presented to show the image quality. The effects of the cross-correlation coefficient and the number of source wavelengths on the imaging results were explored. The value of cross-correlation threshold determines the required time for imaging, the resulted number of tissue groups, and the variety of tissue colors in the imaging result. Owing to its virtual noninvasiveness and easy configuration, the SCI system is highly promising for practical uses. RGB LEDs possess merits of broad bandwidth, low cost, long lifetime, small volume, and are ready to be integrated into a multi-color source module. Replacing the wide-band light source and the spectrometer module with a composite RGB LED with discrete wavelengths and a micro-spectrometer for spectra retrieval, the system has great potential to be minimized as a hand-held product for noninvasive medical imaging. It leads to reduced use of non-eco-friendly cosmetics and extended advance of cosmetic dermatology.
|
Received: 2013-03-17
Accepted: 2013-05-30
|
|
Corresponding Authors:
YANG Bor-wen
E-mail: bwyang@must.edu.tw
|
|
[1] Sheppard C J R, Shotton D M. Confocal Laser Scanning Microscopy. New York: Springer, 1997. [2] Gu M. Principle of Three-Dimensional Imaging in Confocal Microscopes. Singapore: World Scientific, 1996. [3] Zipfel W R, Williams R M, Webb W W, et al. Proc. Natl. Acad. Sci. USA, 2003, 100: 7075. [4] Moreaux L, Sandre O, Mertz J. J. Opt. Soc. Am. B, 2000, 17(10): 1685. [5] Denk W, Strickler J H, Webb W W. Two-Photon Laser Scanning Fluorescence Microscopy. Science, 1990, 248: 73. [6] Diaspro A. Confocal and Two-Photon Microscopy: Foundations, Applications and Advances. New York: Wiley-Liss, 2001. [7] Huang D, Swanson E A, Fujimoto J G, et al. Science, 1991, 254: 1178. [8] Fujimoto J G, Farkas D. Biomedical Optical Imaging. New York: Oxford University Press, 2009. [9] Tuchin V V. Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis. New York: SPIE Optical Engineering Press, 2000. [10] Cheng D K. Field and Wave Electromagnetics. New York: Addison-Wesley, 2005. [11] YANG Bor-wen, LIN Yu-min, WANG Shih-yuan, et al(杨伯温, 林育民, 王诗渊, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(12): 3299. [12] Yang B W, Chan L M, Wang K C. Opt. Rev., 2009, 16(3): 392. [13] Yang B W, Chen X C. Biomed. Opt. Express, 2010, 1(5): 1341. [14] Podoleanu A G, Dobre G M, Webb D J, et al. Opt. Lett., 1997, 22(13): 1039. [15] Yang B W. A Portable Skin Imaging Device Employing Multi-Wavelength Optical Source and Micro-Spectrometer Sensor. Taiwan Patent. In Application.
|
[1] |
HE Qing-yuan1, 2, REN Yi1, 2, LIU Jing-hua1, 2, LIU Li1, 2, YANG Hao1, 2, LI Zheng-peng1, 2, ZHAN Qiu-wen1, 2*. Study on Rapid Determination of Qualities of Alfalfa Hay Based on NIRS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3753-3757. |
[2] |
LIU Wei1, 2, ZHANG Peng-yu1, 2, WU Na1, 2. The Spectroscopic Analysis of Corrosion Products on Gold-Painted Copper-Based Bodhisattva (Guanyin) in Half Lotus Position From National Museum of China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3832-3839. |
[3] |
LIU Wen-bo, LIU Jin, HAN Tong-shuai*, GE Qing, LIU Rong. Simulation of the Effect of Dermal Thickness on Non-Invasive Blood Glucose Measurement by Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2699-2704. |
[4] |
YAN Xue-jun1, ZHOU Yang2, HU Dan-jing1, YU Dan-yan1, YU Si-yi1, YAN Jun1*. Application of UV-VIS Diffuse Reflectance Spectrum, Raman and
Photoluminescence Spectrum Technology in Nondestructive
Testing of Yellow Pearl[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1703-1710. |
[5] |
CHEN Xiao-li1, LI You-li1, LI Wei3, WANG Li-chun1, GUO Wen-zhong1, 2*. Effects of Red and Blue LED Lighting Modes on Spectral Characteristics and Coloring of Tomato Fruit[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1809-1814. |
[6] |
YAN Jun1, FANG Shi-bin1, YAN Xue-jun1, SHENG Jia-wei2, XU Jiang1, XU Chong3, ZHANG Jian2*. Study on the Common Effect of Heat Treatment, Dyeing or Irradiation Treatment on UV-Vis Diffuse Reflectance Spectra of Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3697-3702. |
[7] |
FANG Shi-bin1, JIANG Yang-ming1, YAN Jun1, 2, YAN Xue-jun1, ZHOU Yang3, ZHANG Jian2*. The Types of UV-Vis Diffuse Reflectance Spectra of Common Gray Pearls and Their Coloring Mechanism[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3703-3708. |
[8] |
WU Meng-ruo, QIN Zhen-fang, HAN Liu-yang, HAN Xiang-na*. Preparation and Spectra Study of Artificially Degraded Waterlogged Wood[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2941-2946. |
[9] |
WANG Jing1, 2*, CHEN Zhen3, GAO Quan-zhou1. Diffuse Reflectance Spectroscopy Study of Mottled Clay in the Coastal
Area of Fujian and Guangdong Provinces and the Interpretation of Its
Origin and Sedimentary Environment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2494-2498. |
[10] |
ZHU Meng-yuan1, 2, LÜ Bin1, 2*, GUO Ying2. Comparison of Haematite and Goethite Contents in Aeolian Deposits in Different Climate Zones Based on Diffuse Reflectance Spectroscopy and Chromaticity Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1684-1690. |
[11] |
WENG Shi-zhuang*, CHU Zhao-jie, WANG Man-qin, WANG Nian. Reflectance Spectroscopy for Accurate and Fast Analysis of Saturated
Fatty Acid of Edible Oil Using Spectroscopy-Based 2D Convolution
Regression Network[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1490-1496. |
[12] |
WANG Chun-juan1, 2, ZHOU Bin1, 2*, ZHENG Yao-yao3, YU Zhi-feng1, 2. Navigation Observation of Reflectance Spectrum of Water Surface in Inland Rivers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 878-883. |
[13] |
JIANG Jie1, YU Quan-zhou1, 2, 3*, LIANG Tian-quan1, 2, TANG Qing-xin1, 2, 3, ZHANG Ying-hao1, 3, ZHANG Huai-zhen1, 2, 3. Analysis of Spectral Characteristics of Different Wetland Landscapes Based on EO-1 Hyperion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 517-523. |
[14] |
XU Zhao-jin, LI Dong-liang, SHEN Li*. Study on Diffuse Reflection and Absorption Spectra of Organic and Inorganic Chinese Painting Pigments[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3915-3921. |
[15] |
WANG Cong1, Mara Camaiti2, TIE Fu-de1,3, ZHAO Xi-chen4, CAO Yi-jian5*. Preliminary Study on the Non-Invasive Characterization of Organic Binding Media Employing a Portable Hyperspectral Sensor[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2886-2891. |
|
|
|
|