| 光谱学与光谱分析 |
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| Fluorescence Spectroscopic Study on the Biointeraction of New Organometallic Carborane Derivatives with Bovine Serum Albumin |
| WU Chun-hui1,3, YE Hong-de2, WU De-hong2, YAN Hong2, WANG Xue-mei1*, CHEN Jun4 |
1. State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Southeast University, Nanjing 210096, China
2. State Key Lab of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
3. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
4. National Engineering Research Center for Healthcare Devices, Guangdong Institute of Medical Instruments, Guangzhou 510500, China |
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Abstract The biointeractions between a series of new organometallic carborane derivatives and model protein bovine serum albumin (BSA) were investigated by means of fluorescence and synchronous spectroscopy. The observations demonstrate that the ferrocene-carborane conjugates (FcSB1, FcSB2 and FcSBCO) and the ruthenium(Ⅱ)-arene carborane complexes (RuBFc and RuBCOOH) can form a steady complex with BSA and statically quench its fluorescence. The ferrocene-carborane conjugates could remarkably affect the tertiary structure of BSA and induce the microenvironment changes of Trp and Tyr residues from hydrophilic to hydrophobic environment. But the effect of the ruthenium(Ⅱ)-arene carborane complexes on the tertiary structure of BSA is much less. This study would give meaningful insights into the evaluation of the promising biomedical applications of the new carborane derivatives and benefit the development of potential multifunctional metallodrugs.
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Received: 2012-06-12
Accepted: 2012-10-11
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Corresponding Authors:
WANG Xue-mei
E-mail: xuewang@seu.edu.cn
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