| 光谱学与光谱分析 |
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| Analysis and Identification of Geranium by Two-Dimensional Correlation Infrared Spectroscopy |
| SUN Ren-shuang1, JIN Zhe-xiong1, ZHANG Zhe-peng1, XU Chang-hua2, SUN Su-qin2* |
1. Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China
2. Department of Chemistry, Tsinghua University, Beijing 100084, China |
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Abstract Tri-step infrared spectroscopy (Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra and two-dimensional correlation infrared spectroscopy (2D-COS) ) was employed to identify and analyze the main components of Heilongjiang (HLJG), Jilin (JLG), Liaoning (LNG) genuine Herba Geranium. The emergence of several characteristic absorption peaks of tannins including 1 730 and 1 337 cm-1 and peaks around 1 618 and 1 318 cm-1 belonging to calcium oxalate suggested that Herba Geranii contained tannins and calcium oxalate. Differences near 1 370 and 1 230 cm-1 were found among the three Herba Geranii. In light of second derivative spectra, four more peaks of tannin components around 1 509, 1 204, 764 and 763 cm-1 and evident differences around CO stretching bands (1 750～1 600 cm-1) were observed. By 2D-COS spectra with further improved resolution, the three genuine Geraniums were visually distinguished due to their significant differences in auto-peak profile. HLJG has 7 auto peaks with a strongest peak around 1 621 cm-1, while JLG and LNG both have only 4 auto peaks with a strongest peak around 1 580 and 1 659 cm-1, respectively. It was demonstrated that the Tri-step infrared spectroscopy was successfully applied to fast analyze and identify genuine Geraniums from different geographical regions and subsequently would be applicable to the study of Chinese medicinal resources and quality standards.
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Received: 2012-04-17
Accepted: 2012-06-20
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Corresponding Authors:
SUN Su-qin
E-mail: sunsq@tsinghua.edu.cn
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[1] Pharmacopiea Committee of the Ministry of Health of the People’s Republic of China(中华人民共和国国家药典委员会编). Pharmacopiea the People’s Republic of China(中华人民共和国药典). Beijing: The Medicine Science and Technology Press of China(北京：中国医药科技出版社)， 2010. 80.
[2] XIAO Pei-gen(肖培根). Modern Chinese Materia Medica(新编中药志). Beijing: Chemical Industry Press(北京：化学工业出版社)，2002.
[3] XUE Jian-ying(薛建英). China Herbal Medicine(中药材)，1989, 12(8)：11.
[4] DU Xiao-ming, GUO Yong(杜晓鸣，郭 永). World Notes on Plant Medicine(国外医学植物药分册)，1990，5(2)：762.
[5] LEI Hai-min,WEI Lu-xue(雷海民，魏璐雪). Northwest Pharmaceutical Journal(西北药学杂志)，1997，12(5)：207.
[6] GUO Jia-jia, WANG Su-shen, LI Xi, et al(郭佳佳，王素肾，李 铣，等). Acta Pharmaceutica Sinica(药学学报)，1987，22(1)：28.
[7] LIU Dai-lin, HU Ying-qing, LIU Cheng-hang, et al(刘岱琳，胡迎庆，刘成航, 等). Medical College of Chinese People’s Armed Police Forces (武警医学)，2003，14(11)：l8.
[8] FENG Ping-an, JIA De-yun, LIU Chao(冯平安，贾德云，刘 超). Clinical Journal of Traditional Chinese Medicine(安徽中医临床杂志)，2003，15(6)：511.
[9] HU Ying-qing, LIU Dai-lin, ZHOU Yun-chou(胡迎庆，刘岱琳，周运筹，等). Northwest Pharmaceutical Journal(西北药学杂志)，2003，18(3)：113.
[10] SUN Su-qin, ZHOU Qun, QIN Zhu(孙素琴，周 群，秦 竹). Atlas of Two-Dimensional Correlation Infrared Spectroscopy for Traditional Chinese Medicine Identification(中药二维相关红外光谱鉴定图集). Beijing: Chemical Industry Press(北京：化学工业出版社), 2003.
[11] SUN Su-qin, ZHOU Qun, CHEN Jian-bo(孙素琴，周 群，陈建波). Analysis of Traditional Chinese Medicine by Infrared Spectroscopy(中药红外光谱分析与鉴定). Beijing: Chemical Industry Press(北京：化学工业出版社), 2010.
[12] ZHOU Qun, LI Jing, SUN Su-qin(周 群，李 静，孙素琴，等). Chinese Journal of Analytical Chemistry(分析化学)，2003，31(3)：292. |
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