| 光谱学与光谱分析 |
|
|
|
|
|
| Study on Application of Multi-Wavelength LED Array Induced Fluorescence Spectrum in Multicomponent Analysis |
| SIMA Wei-chang1,ZHANG Yu-jun1*,WANG Zhi-gang2, LI Hong-bin1, ZHAO Nan-jing1, LU Zhi-xian1, LIU Wen-qing1 |
1. Key Lab of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China
2. School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China |
|
|
|
|
Abstract The technique of fluorescence spectrum is one of the efficient methods for monitoring and identifying dissolved organic matter, oil pollutants and biomass of phytoplankton in water. A new kind of fluorescence spectrum system with a multi-wavelength LED array as the exciting light source is introduced. The principle of the system and the method for multi-component analysis are discussed. The excitation-emission matrix (EEM) fluorescence spectra of samples mixed with different concentration fluorescent dyes were measured with this system in the authors’ laboratory, which were analyzed using parallel factor (PARAFAC) algorithm. The results indicate that the correlation coefficient of the resolved concentration and actual ones is up to 98%, which shows the potential application of the fluorescence spectrum system in multi-component analysis.
|
|
Received: 2006-09-08
Accepted: 2006-12-18
|
|
|
|
Corresponding Authors:
ZHANG Yu-jun
E-mail: yjzhang@aiofm.ac.cn
|
|
[1] www. fluorometer. com，SCUFA brochure. pdf.
[2] Beutler M, Wiltshire K H, Meyér B, et al. Photosynthesis Research，2002，72(1)：39.
[3] ZHANG Biao, CHU Yan-nan, ZHANG Yu-ping, et al(张 彪，储焰南，张玉平，等). Chinese Journal of Quantum Electronics(量子电子学报)，2003，20(4)：472.
[4] SONG Ji-mei, TANG Bi-lian(宋继梅，唐碧莲). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2000，20(1)：115.
[5] CHEN Guo-zhen, HUANG Xian-zhi，ZHENG Zhu-zi, et al(陈国珍，黄贤智，郑朱梓，等). Fluorescence Analytical Method(荧光分析法). Beijing：Science Press(北京: 科学出版社)，1990.
[6] Michelle L Nahorniak，Karl S Booksh, et al. J. Chemometrics，2003，17：608.
[7] NI Yong-nian，CAI Ying-jun(倪永年，蔡英俊). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(10)：1641.
[8] Hart Sean J，JiJi Renee D, et al. Analyst，2002，127：1694.
[9] LI Hong-bin，LIU Wen-qing，WANG Zhi-gang, et al(李宏斌，刘文清，王志刚，等). Chinese Journal of Quantum Electronics(量子电子学报)，2007，24(3)：306.
[10] ZHANG Qian-qian，LEI Shu-he，WANG Xiu-lin, et al(张前前，类淑河，王修林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(10)：1227. |
| [1] |
CHEN Xiao-yu1, ZHANG kun1, KONG De-ming2*. Three-Dimensional Fluorescence Partial Derivative Spectroscopy Combined With Parallel Factor Algorithm for Detection of Mixed Oil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3506-3511. |
| [2] |
ZHANG Qiang1, 2, WEI Ru-yi1*, YAN Qiang-qiang1, ZHAO Yu-di1, ZHANG Xue-min1, YU Tao1. Application of Deep Neural Network in Quantitative Analysis of VOCs by Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1099-1106. |
| [3] |
WANG Shu-tao*, LI Ming-shan, WANG Yu-tian, WU Xing, CHENG Qi, CHE Xian-ge, ZHU Wen-hao. Qualitative and Quantitative Analysis of PAHs Based on Three-Dimensional Fluorescence Spectroscopy and PARAFAC[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(02): 494-500. |
| [4] |
SUN You-wen，LIU Wen-qing*，WANG Shi-mei，HUANG Shu-hua，YU Xiao-man . Research on the Method of Interference Correction for Nondispersive Infrared Multi-Component Gas Analysis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(10): 2719-2724. |
| [5] |
ZHANG Lin1,ZHANG Li-ming1,LI Yan1*,WANG Xiao-fei1，2,HU Lan-ping1，３,WANG Jun-de1 . Multi-Component Analysis of FTIR Spectra of Non-Linear System Using Polynomial Partial Least Squares Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 620-623. |
| [6] |
LIU Bing-ping1，2, LI Yan1*, ZHANG Lin1, ZHANG Li-ming1, WANG Xiao-fei1，3, WANG Jun-de1 . Automated Recognition of VOCs Using Artificial Neural Networks [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 51-53. |
|
|
|
|
