光谱学与光谱分析 |
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Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System |
TIAN Ning1, ZHANG Lu2, ZHENG Jing-hao1, LI Ying2, MA Wan-yun1* |
1. State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China 2. Department of Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China |
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Abstract Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors’ results showed that aging mouse oocytes may be not suitable for in-vitro maturation: both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA methylation modification.
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Received: 2012-04-19
Accepted: 2012-07-05
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Corresponding Authors:
MA Wan-yun
E-mail: mawy@tsinghua.edu.cn
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