| 光谱学与光谱分析 |
|
|
|
|
|
| Steady-State Spectroscopy on Complementary Tissue Structural and Functional Information Extraction |
| LIU Cheng-bo, SONG Jing-bo, LONG Tao, ZHANG Zhen-xi* |
| The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China |
|
|
|
|
Abstract Steady-state spectroscopy is prevailing for complementary tissue structural and functional information extraction. The goal of the present study is to experimentally validate the merit of this technique for optical properties and intrinsic fluorescence extraction from tissue mimicking phantoms. Phantom studies were carried out in which HbO2 was used as absorber, polystyrene sphere was used as scatterer, and stilbene was used for fluorescence emission. Two sets of phantoms corresponding to two absorption levels and one scattering level were made, and 4 fluorophore concentrations were titrated in each set. The results indicate that both optical properties and intrinsic fluorescence can be extracted with high accuracy, implying that it’s feasible to extract complementary structural and functional information with steady-state spectroscopy.
|
|
Received: 2012-03-19
Accepted: 2012-06-20
|
|
|
|
Corresponding Authors:
ZHANG Zhen-xi
E-mail: zxzhang@mail.xjtu.edu.cn
|
|
[1] Sokolov K, Follen M, Richards-Kortum R. Current Opinion in Chemical Biology, 2002, 6: 651.
[2] Shahzad A, Knapp M, Edetsberger M, et al. Applied Spectroscopy Reviews, 2010, 45: 92.
[3] Hillemanns P, Weingandt H, Baumgartner R, et al. Cancer, 2000, 88: 2275.
[4] Sterenborg H J C M, Motamedi M, Wagner R F, et al. Lasers in Medical Science, 1994, 9: 191.
[5] Peters V G, Wyman D R, Patterson M S, et al. Physics in Medicine and Biology, 1990, 35: 1317.
[6] Palmer G M, Ramanujam N. Journal of Biomedical Optics, 2008, 13: 024017.
[7] Chang V T, Cartwright P S, Bean S M, et al. Neoplasia, 2009, 11: 325. |
| [1] |
YU Yang1, ZHANG Zhao-hui1, 2*, ZHAO Xiao-yan1, ZHANG Tian-yao1. Study on Extraction Method of Terahertz Spectral Parameters of Rough Surface Samples[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 386-391. |
| [2] |
NIE Min1, LU Chun-lei1*, LIU Meng1, YANG Guang1, 2, PEI Chang-xing3. Study of Nerve Cell Localization after Spinal Cord Injury Based on Monte Carlo Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1433-1438. |
| [3] |
LI Chen-xi1,2, JIANG Jing-ying2, LIU Rong1,2, CHEN Wen-liang1, 2*, XU Ke-xin1,2. Research on the Influence of Pressure-Induced Skin Deformation on the Diffuse Reflectance Spectra Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(10): 3006-3010. |
| [4] |
HU Wen-yan1,3, WANG Yu-heng1,3, ZHANG Xiao-shuan2, LI Jun-hui1,3, WANG Zhong-yi1,3, ZHAO Long-lian1,3*. Determination and 3D Reconstruction of Optical Parameters of Biological Tissue Based on Frequency-Domain Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1374-1379. |
| [5] |
LIU Yong1,2, ZHANG Yuan-zhi1, HOU Hua-yi1, ZHU Ling1,2, WANG An1, WANG Yi-kun1,2*. Tissue Intrinsic Fluorescence Spectrum Recovering Based on Diffusion Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(12): 3836-3841. |
| [6] |
LIU En-chao, LI Xin, WEI Wei, ZHAI Wen-chao, ZHANG Yan-na, ZHENG Xiao-bing . Automatic Field Calibration and Analysis of Satellite Based on Hyper-Spectral Ratio Radiometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(12): 4076-4081. |
| [7] |
HAN Xiao-hui, ZHANG Jin*, YANG Ye, MA Yu-ting, CHANG Tian-ying, CUI Hong-liang . Review on the Methodology for Optical Parameter Extraction with Terahertz Time-Domain Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(11): 3449-3454. |
| [8] |
LI Chen-xi1, 2, SUN Zhe2, HAN Lei2, ZHAO Hui-juan1, 2*, XU Ke-xin1, 2. Study on the Determination System of Tissue Optical Properties Based on Diffuse Reflectance Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(05): 1532-1536. |
| [9] |
HAN Lei1, LI Chen-xi1, 2*, SUN Cheng-tao1, JIANG Jing-ying1, ZHAO Hui-juan1,2, XU Ke-xin1,2 . The Research on Measurement System and Method of Tissue Optical Parameters with Wide Spectra Based on Double-Integrating-Spheres[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 561-566. |
| [10] |
MA Feng-ying, CHI Quan, SU Jian-po*, DU Yan-li, ZHANG Wei-wei, CHEN Ming, LIU Jian-li, GUO Mao-tian, YUAN Bin . Study on the Optical Properties of Ultra-Thin Metal Films in the THz Band[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(03): 610-613. |
| [11] |
LIU Chang, WANG Xin-ke, SUN Wen-feng, ZHANG Yan* . Accurate Optical Parameters Extracting of Non-Polar Organic Solvents in the Terahertz Range [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(11): 2886-2890. |
| [12] |
LIU Li,HUANG Lan,YAN Yan-lu,WANG Zhong-yi*. Progress in the Study of Impact of Scattering on Stability of Quantitative Analysis Model Using Near Infrared Spectroscopy Technology and Correction Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(10): 2290-2295. |
| [13] |
ZHAO Jun1, DING Hai-shu1*，HOU Xin-lin2, ZHOU Cong-le2 . Non-Invasive Determination of the Optical Properties of Neonatal Brain [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(11): 1768-1771. |
|
|
|
|
