| 光谱学与光谱分析 |
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| Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) inside Living Human Lung Adenocarcinoma Cells |
| CHEN Tong-sheng1,WANG Xiao-ping2,WANG Zhi-ping3,WANG Long-xiang1,WANG Hui-ying1,XING Da1 |
1. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China
2. Department of Anesthesiology, The First Affiliated Hospital of Ji’nan University, Guangzhou 510632, China
3. Science and Technology Park Ltd., Guangzhou University of TCM, Guangzhou 510445, China |
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Abstract The CCK-8 was used to measure the inhibition effect of Xiao-Ai-Ping (XAP), a traditional medicine, on the human lung adenocarcinoma (ASTC-a-1) cells viability. The ASTC-a-1 cells expressing stably with SCAT3, a fluorescence resonance energy transfer (FRET) plasmid based on the green fluorescent protein mutants (GFPs), was verified using confocal fluorescence scanning microscopy imaging, fluorescence emission spectra and FRET acceptor photobleaching techniques. The caspase-3 activation can be monitored by the fluorescence emission spectra of SCAT3 inside living cells. The cells expressing stably with SCAT3 were cultured with XAP for 96 hours, and the fluorescence emission spectra of the SCAT3 inside living cells were measured at the time of 0, 24, 72, and 96 hours, respectively. Experimental results showed that：（1）XAP inhibited obviously the proliferation of ASTC-a-1 cells and induced the cell death. The inhibition of XAP on the cells was dose-dependent；（2）the SCAT3 inside living cells was cleaved completely 72 hours after the XAP treatment, implying that a great deal of pro-caspase-3 was activated by XAP；（3）24 hours after XAP treatment, the emission spectra of SCAT3 inside living cells cultured in DMEM without XAP for 48 and 72 hours did not change greatly, implying that XAP did not activate obviously caspase-3 within 24 hours.
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Received: 2007-01-18
Accepted: 2007-04-19
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Corresponding Authors:
CHEN Tong-sheng
E-mail: chentsh@scnu.edu.cn
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[1] LI Mao-quan, SHEN Jian-hua, XU Bin, et al(李茂全，沈建华, 胥 彬，等). J. Interventional Radiology(介入放射性杂志), 2001, 10(4)：228.
[2] SUN Yu, SHEN Jian-hua, ZHU Mei-hua, et al(孙 珏, 沈建华, 朱美华, 等). Acta University Traditions Medicalis Sinensis Pharmacologiaeque Shanghai（上海中医药大学学报）, 2000, 14(2)：41.
[3] YANG Min, WU Jing, LI Yong-qiang, et al(杨 岷，吴 静，李永强，等). Traditional Chinese Medicinal Research(中医研究)，2000，13(1)：39.
[4] XING Wang-xing, CHENG Rong-zhen, LIN Pei-ying, et al(邢旺兴，程荣珍，林培英，等). Acad. Sec. Mil. Med. Univ.(第二军医大学学报)，2003，24(12)：1338.
[5] HUANG Zhen-qian, TAN Huo, WANG Chun-yan, et al(黄振倩，谭 获，王春燕，等). Chinese Clinical Oncology(临床肿瘤学杂志)，2007，12(2)：97.
[6] PEI Yi, WEI Shu-qing, YANG Yong-ming, et al(裴 毅，魏淑青，杨永明，等). Cancer Research and Clinic(肿瘤研究与临床)，2006，18(7)：469.
[7] Nika N D, Stanley J K. Cell, 2004, 116: 205.
[8] Takemoto K, Nagai T, Miyawaki A, et al. Journal Cell Biology, 2003, 160(2)：235.
[9] Onuki R, Nagasaki A, Kawasaki H, et al. Proceedings of the National Academy of Science of the United States of America, 2002, 99: 14716.
[10] GAO Xue-juan, CHEN Tong-sheng, XING Da, et al. Journal of Cellurar Physiology, 2006, 206(2): 441.
[11] WANG Fang, CHEN Tong-sheng, XING Da, et al. Laser in Surgery and Medicine, 2005，36：2.
[12] LIN Dan-ying，SUN Yun-xu，MA Wan-yun，et al(林丹樱, 孙云旭, 马万云, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006，26（5）：917.
[13] WANG Jin, WANG Jing, SUN Yi-hong, et al(王 进，王 晶，孙依红，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(2)：279.
[14] WANG Wei, FANG Qi, LIU Zhi-qiang, et al(王 伟，方 奇，刘志强，等). Acta Chimica Sinica(化学学报)，2005，63(14)：1323.
[15] Zhang C, Wang C, Deng M, et al. Biochem. Biophys. Res. Commun., 2002, 290(5): 1411.
[16] Alessandra P, Sylvia D, Marco N, et al. Structure, 2003, 11(9): 1087.
[17] Alessandra P, Sylvia D, Marco N, et al. Micron, 2004, 35(1-2): 63.
[18] LI Bu-hong，XIE Shu-sen（李步洪，谢树森）. Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(7)：1083.
[19] CHEN Li-hua, YIN Hong, YANG Zhao-xia, et al(陈莉华, 尹 红, 杨朝霞, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006, 26(9)：1640.
[20] XU Chun-yan, HUANG Ming-zhi, XUE Chuan-xin(徐春艳，黄明智，薛传薪). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25 (11)： 1772.
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