| 光谱学与光谱分析 |
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| Nonlinear Spectral Imaging of DNA Specimens Derived from Tumor Cells Based on Second Harmonic Generation |
| LUO Dong-mei1, DENG Xiao-yuan1, ZHUO Shuang-mu2, TAN Shu-wen1, ZHUANG Zheng-fei1, JIN Ying1* |
1. College of Biophotonics of South China Normal University, Guangzhou 510631, China
2. Key Laboratory of Optoelectronic Science and Technology for Medicine of Fujian Normal University, Fuzhou 350007, China |
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Abstract Second harmonic generation (SHG) is a second-order nonlinear optical process that has symmetry constraints confining signal to regions lacking a center of symmetry. Using SHG microscopy, a variety of tissue structures have noninvasively been imaged by virtue of intrinsic signal generated by structured proteins such as collagen fibrils in connective tissues or the actomyosin lattice of muscle cells. In biochemistry and structure biology, the high-level structures of DNA and protein macro-molecules are similar in constructing mechanism, although DNAs consist of deoxynucleotides and proteins of amino acid residues. The principal purpose of present work is to detect the SHG signal from different DNA samples by spectral imaging technology based on two-photon excited fluorescence (TPEF) and SHG. These DNA samples include the solution of genomic DNA and extracted nuclei, and cultured living cells. Results show that we can obtain the SHG signal from solution of genomic DNA and extracted nuclei in routine condition, but nothing from cultured cell nuclei. After adding a little of absolute ethanol (less than 5% by volume) in culture medium, the SHG signal is detectable in the interested region of nuclei. The findings suggest that the interaction between ethanol and DNA in living cell gives rise to the shift of molecular conformation, and this shift changes some nonlinear optical properties of DNA molecules.
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Received: 2011-07-11
Accepted: 2011-10-28
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Corresponding Authors:
JIN Ying
E-mail: jinying@scnu.edu.cn
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