| 光谱学与光谱分析 |
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| Synthesis of Sulfur-Contained D(+)-Glucosamine Metal Complexes and Interaction with DNA and Serum Albumin |
| WU Shan-shan1,ZHANG Yin-feng2,DU Jin-feng1,ZHANG Qi1*, YUAN Wen-bing1,GU Hai-bo1 |
1. Hainan Provincial Key Lab of Fine Chem., Hainan University, Haikou 570228, China
2. School of Life Sciences, University of Science and Technology of China, Hefei 230027, China |
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Abstract Four sulfur-contained D(+)-glucosamine metal complexes were synthesized (M-GLUS，M = Co, Cu, Ni and Zn)and characterized by elementary analysis, molar conductance, and proton nuclear magnetic resonance. The yield of the complex was about 70%, and it dissolved easily in water. The ligand coordinates with metalions mainly between sulphur and metalions, the coordination molar ratio of the ligand to metalion was 2∶1, and the molar conductivity indicated that all the complexes are nonelectrolyte. The mechanism of the interaction between metal complexes and calf thymus (ct) DNA in Tris buffer (pH=7.08), was studied by ultraviolet absorption and fluorescence spectroscopy. The results from varied experiments showed that the intensity of the maximal absorption peaks increased with gradual addition of metal complexes, but the metalions can decrease the maximal absorption and the ligand has no effect on it. Meanwhile, metal complexes could remarkably quench the emission intensity of the DNA-EB system, and the metal complexes could be bound to ct DNA. The quenching mechanism was discussed by Stern-Volmer’s equation, the figure showed that it is influenced by static quenching and dynamic quenching, so the partial interaction of the complexes and ct DNA was the major mode. Under physiological pH condition, this study was designed to examine the effect of complexes on human serum albumin and bovine serum albumin by fluorescence. The binding constants and sites of the interaction with SA were analyzed by the Scatchard’s equation, the results indicated that there was a strong interaction between the four metal complexes and serum albumin and the binding force was Co-GLUS＞Zn-GLUS＞Cu-GLUS＞Cu-GLUS, and the binding site is only one.
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Received: 2007-02-28
Accepted: 2007-05-29
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Corresponding Authors:
ZHANG Qi
E-mail: hnfinechem@163.com
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