| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Interactions of Eu(Ⅲ) Complex of O-(Thioxanthone-[2]-yl)-Oxyacetic Acid with DNA |
| YANG Wu,GAO Qi-kuan,LU Xiao-lin,WU Jin-xiu |
| College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China |
|
|
|
|
Abstract The interaction of the Eu(Ⅲ) complex of O-(thioxanthone-[2]-yl)-oxyacetic acid and calf thymus DNA was studied using fluorimetric, UV-Vis and CD(circular dichroism) methods. It was found that the ultraviolet absorption intensity of Eu(Ⅲ) complex of O-(thioxanthone-[2]-yl)-oxyacetic acid decreased as the concentration of DNA increased under the physiological condition of pH 7.10, and the compound remarkably influenced the negative peak in CD spectra of DNA when Eu(Ⅲ) complex of O-(thioxanthone-[2]-yl)-oxyacetic acid interacted with DNA. The emission intensity of the Eu(Ⅲ) complex evidently increased in the presence of DNA. The emission intensity of the DNA-EB system decreased as the concentration of the complex increasesd. And the presence of EB also led to the decrease in the emission intensity of the DNA-complex system. These changes observed here are often characteristic of intercalation. The above results strongly suggest that the complex can bind to DNA mainly by intercalation.
|
|
Received: 2006-09-08
Accepted: 2006-12-18
|
|
|
|
Corresponding Authors:
YANG Wu
E-mail: yangw@nwnu.edu.cn
|
|
[1] Shanta Dhar, Munirathinam Nethaji, Akhil R Chakravarty. Inorganic Chim. Acta,2005, 358: 2437.
[2] JI Liang-nian, ZHANG Qian-ling, CHAO Hui(计亮年, 张黔玲，巢 晖). Chinese Science Bulletin(科学通报)，2001, 46: 451.
[3] WANG Zhong-ming, JI Liang-nian(汪中明，计亮年). Progress in Chemistry(化学进展),2002, 14: 296.
[4] YANG Pin, SONG Yu-fei(杨 频，宋宇飞). Progress in Chemistry(化学进展),2000, 12: 32.
[5] SONG Yu-fei, YANG Pin(宋宇飞, 杨 频). Progress in Chemistry(化学进展),2001，13: 368.
[6] Kumar C V, Asuncion E H. J. Am. Chem. Soc., 1993, 115: 8547.
[7] Franklin S J, Curr. Opin. Chem. Biol.,2001, 5: 201.
[8] SONG Yu-min, YANG Pei-ju, WANG Liu-fang, et al(宋玉民, 杨培菊，王流芳, 等). Acta Chimica Sinica(化学学报),2003, 61: 1266.
[9] Sydaey Archer, Abdel-Hadizayed, Rabindra Rej, et al. J. Med. Chem., 1983,26: 1240.
[10] TANG Hui-an, WANG Liu-fang(唐慧安，王流芳). Journal of Lanzhou University(Natural Science)(兰州大学学报·自然科学版),2001，37: 143.
[11] SONG Yu-min, LU Xiao-lin, TANG Lui-an, et al(宋玉民，芦小林，唐慧安，等). Acta Chimica Sinica(化学学报),2006, 64: 1451.
[12] DU Jiang-yan, HUANG Xiao-hua, XU Fei, et al(杜江燕，黄晓华，徐 飞，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(9): 1435.
[13] ZHANG Fang, ZHANG Qian-qian, ZHU Chen-jian, et al(张 芳，张前前，祝陈坚，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(9): 1439.
[14] Pasternack P F, Gibbs FJ, Villatrnaca J. Biochem., 1983,22: 2406.
[15] Rehmann J P, Barton J K. Biochemistry, 1990,29: 1701.
[16] JIN Lan, YANG Pin(靳 兰, 杨 频). Chemical Research in Chinese Universites(高等学校化学学报), 1996, 17: 1345.
[17] LU Ji-xin, ZHANG Gui-zhu, HUANG Zhi-na, et al(卢继新，张贵珠，黄志娜，等). Acta Chimica Sinica(化学学报),2002, 60: 967.
[18] LIU Yong-ming, LI Gui-zhi, JING Ji-rong(刘永明，李桂芝，荆济荣). Chin. J. Anal. Chem.(分析化学),2002, 30(11): 1341. |
| [1] |
WU Cheng-ke1, DONG Qiu-peng1，2, ZHANG Hong1, XIONG Cen1, WU Xiu-li1, LING Lian-sheng1* . Spectral Study on the Interaction of [Cu(DPPZ)(L-Ser)]+ Complex with DNA and Its Analytical Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(04): 1057-1060. |
| [2] |
TONG Chang-lun, ZHANG Xiao-xiang. Study on the Interaction of Ofloxacin and Levofloxacin with DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 481-485. |
| [3] |
DUAN Yun-qing1,2,MIN Shun-geng1* . Study of the Interaction Mechanism between Brodifacoum and DNA by Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(04): 999-1003. |
| [4] |
MA Jing2, MENG Qing-xiang1, REN Li-ping1*, ZHANG Tao2, MU Hao-jie2. Study on the Interaction of Atrazine and ct-DNA by Fluorescence Probe Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2122-2125. |
| [5] |
LI Hua1,YU Yan-ying1*,HU Xin1,CAO Shu-wen1,2. Research on the Interactions between Genistein and Its Glucosides with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(08): 1905-1909. |
| [6] |
YU Yan-ying1,LI Hua1,HU Xin1,CAO Shu-wen2*. Research on the Interaction of Cr(Ⅲ) Complex of Genistein with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(07): 1587-1591. |
| [7] |
WU Shan-shan1,ZHANG Yin-feng2,DU Jin-feng1,ZHANG Qi1*, YUAN Wen-bing1,GU Hai-bo1. Synthesis of Sulfur-Contained D(+)-Glucosamine Metal Complexes and Interaction with DNA and Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 374-379. |
| [8] |
HENG Hang,KE Wei-zhong*,JI Kang. Spectral Analysis of DNA Solution Treated with Acid at Various pH for Different Time[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 932-935. |
| [9] |
ZHANG Xiao-ming1,LI Guo-xue2,LI Dong-hao1,MIN Shun-geng1,SUN Ying1* . Interaction between Carbaryl and Calf Thymus DNA Enhanced via Cu2+[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 517-520. |
| [10] |
GAN Ning1, 2, GE Cong-xin1. Study of Interaction of Ciprofloxacin Probe and Calf Thymus DNA Using Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2276-2279. |
| [11] |
LIU Xue-ping,FENG Su-ling*, PAN Zi-hong, FAN Jing . Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1895-1898. |
| [12] |
CHEN Chang-yun1，3, MA Mei-hua1, ZHOU Zhi-hua2, LU Kang1, XIANG Bing-ren3 . Spectroscopic Studies on the Binding of Prulifloxacin and ct-DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1891-1894. |
| [13] |
WANG Ping-hong1, ZHANG Qi1*, YUAN Wen-bing1, WANG Liu-fang2, SONG Yu-min3, LIU Hui-hong1 . Studies on the Mechanism of the Interaction between Hydrazide-Podophyllic Ni(Ⅱ), Co(Ⅱ), Zn(Ⅱ) Metal Complexes and DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1298-1302. |
| [14] |
WANG Ping-hong1, ZHANG Qi1*, WANG Liu-fang2, SONG Yu-min3, QU Jian-qiang2, LIU Ying-qian2 . Studies on the Interaction of the Metal Complex of Hydrazide of Podophyllic Acid with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 941-943. |
| [15] |
TANG Ling-tian, WANG Yi, LIU Xin-qi，HU Shao-wen, CHU Tai-wei, WANG Xiang-yun*. Spectroscopic Research on the Interaction of 2-(4-Dimethylaminophenyl)-5-Fluoro-6-(Morpholin-4-yl)-1H-Benzimidazole and Calf Thymus DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1618-1621. |
|
|
|
|
