光谱学与光谱分析 |
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Investigation on Low Power Microwave Irradiation-Assisted Enzymatic Esterification in Organic Solvent by Fluorescence Spectroscopy |
MIN Rui1,FANG Yun1,XIA Yong-mei1,2* |
1. School of Chemical & Materical Engineering,Jiangnan University, Wuxi 214122, China 2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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Abstract The authors studied the fluorescence change of immobilized lipase from Rhizomucor miehei in the microwave assisted enzymatic esterification of caprylic acid and butanol in organic medium by investigating the fluorescence spectra in solvent or aqueous buffer after incubating the lipase with the solvent, caprylic acid and butanol under microwave irradiation, respectively. A comparison was made with the conventional heated enzymatic esterification in the solvents. Both of the heating modes, the microwave irradiation and conventional heating, can enhance the fluorescence intensity without shifting the emission wavelength of the lipase. In the circumstance that the irradiation can accelerate the esterification, the irradiation can enhance the exposure of the lipase protein molecules in the aqueous environment after incubating the lipase with solvents or the substrates. The effect of the reaction mixture on the fluorescence intensity was dominated by the solvents. The trend of the plot of log P versus the initial reaction rate was similar to that of log P versus fluorescence intensity of lipase in aqueous buffer after esterification;but was different from that of log P versus fluorescence intensity of lipase in organic medium.
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Received: 2007-11-02
Accepted: 2008-02-06
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Corresponding Authors:
XIA Yong-mei
E-mail: ymxia@126.com
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[1] Vanita D Sood, David Baker. Journal of Molecular Biology, 2006, 357(3): 917. [2] Senthilkumar R, Sharma K Krishna. Journal of Protein Chemistry, 2002, 21(5): 324. [3] Yan S L, Liu Y L, Tian X J, et al. Journal of Protein Chemistry, 2003, 22(4): 371. [4] ZHANG Qiang, YUAN Jing-ming(张 强, 袁静明). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1998, 18(1): 30. [5] YANG Yu-hong, SHAO Zheng-zhong, CHEN Xin(杨宇红, 邵正中, 陈 新). Journal of the Chinese Chemical Society(化学学报), 2006, 64(16): 1730. [6] Zhong K, Wu J H, Wang Z F, et al. Food Chemistry, 2007, 100: 115. [7] DENG Yi-bing, YANG Ti-qiang(邓一兵, 杨体强). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(7): 1312. [8] Jocelyn A Laurence, Peter W French, Robyn A Lindner, et al. J. Theor. Biol, 2000, 206: 291. [9] DENG Hua, SONG Zhan-jun, WANG De-wen, et al(邓 桦, 宋占军, 王德文, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(8): 1437. [10] WU Gen-hua, WANG Chun-hua(吴根华, 汪春华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(2): 246. [11] Huang W, Xia Y M, Gao H, et al. Journal of Molecular Catalysis B: Enzymatic, 2005, 35: 113. [12] Laane C, Boeren S, Vos K, et al. Biotechnology and Bioengineering, 1987, 30: 81. |
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