| 光谱学与光谱分析 |
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| Second Structure of the Protein Factions from Lotus Seeds |
| CAI Lian-hui, ZENG Hong-yan*, CAI Xi-ling, WANG Ya-ju |
| School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China |
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Abstract Following the sequential Osborne extraction procedure, the proteins of lotus seeds were classified. The secondary structures of albumin, globulin, prolamine and glutelin fractions were determined by Fourier transform infrared spectroscopy (FTIR). The FTIR images of amide Ⅰ and Ⅲ bands from the four protein fractions were analyzed using Fourier deconvolution and curve-fitting technique. The results showed that there were minor differences in every corresponding peak position and peak area percent of secondary structure between albumin and globulin as well as between prolamin and glutelin. But there were differences in every corresponding peak position between albumin (or globulin) and prolamin (or glutelin). Especially the area percents of the corresponding nonrandom structures (α-helix and β-sheet) of albumin and globulin were significantly larger than those of prolamin and glutelin. The contents of nonrandom structures of albumin and globulin extracted with 0.1 mol·L-1 NaCl solution were about 55% and those of prolamine and glutelin fractions were only at round 40%, indicating that the secondary structures of the salt-extraction protein were ordered and stable.
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Received: 2010-12-16
Accepted: 2011-03-30
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Corresponding Authors:
ZENG Hong-yan
E-mail: hyzeng@xtu.edu.cn
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[1] CAI Lian-hui, ZENG Hong-yan, WANG Ya-ju, et al(蔡联辉, 曾虹燕, 王亚举, 等). Acta Nutrimenta Sinica(营养学报), 2010, 32(5): 503.
[2] TANG Pei-hua, JIANG Zai-jie, MEI Chu-hong, et al(唐佩华, 姜在阶, 梅楚红, 等). Journal of Beijing Normal University·Natural Science(北京师范大学学报·自然科学版), 1998, 34: 532.
[3] ZHANG Yu, ZHENG Tie-song, CHEN Jing(张 羽, 郑铁松, 陈 静). Food Science(食品科学), 2007, 28: 144.
[4] Naumann D, Labischinski H, Giesbrecht P. Modern Techniques for Rapid Microbiological Analysis. New York, USA: VCH Publishers，1991. 43.
[5] Kong J L, Yu S N. Acta Biochimica et Biophysica Sinica，2007, 39(8): 549.
[6] Zhao X Y, Chen F S, Xue W T, et al. Food Hydrocolloids，2008, 22(4): 568.
[7] Palaniappan PL RM, Vijayasundaram V. Romanian Journal of Biophysic, 2008, 18(2): 135.
[8] FAN Lu, WANG Mei-mei, YANG Hong-wei, et al(范 璐, 王美美, 杨红卫, 等). Chinese Journal of Analytical Chemistry(分析化学), 2007, 35(3): 390.
[9] Choi S M, Ma C Y. Food Chemistry，2007, 102: 150.
[10] Lin S Y, Li M J, Wei Y S. Spectrochimica Acta Part A，2004, 60: 3107.
[11] Fu K, Griebenow K, Hsieh L, et al. Journal of Controlled Release，1999, 58: 357.
[12] XIE Meng-xia, LIU Yuan(谢孟峡, 刘 媛). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24(2): 226. |
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