光谱学与光谱分析 |
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Study on the Structure of Defatted Wheat Germ Protein Isolate under Ultrasonic Treatment |
LIU Bin1, 2,MA Hai-le1, 3*,LI Shu-jun1, 2,TIAN Wan-min1,WU Ben-gang1 |
1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China 2. Chinese Academy of Agricultural Mechanization Sciences, Beijing 100083, China 3. Jiangsu Provincial Research Centre of Bio-Process and Separation Engineering of Agri-Products, Zhenjiang 212013, China |
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Abstract The structure changes of defatted wheat germ protein Isolate (DWGP) treated by ultrasonic was determined by FTIR and fluorescence spectra, and the effect of its structure changes on the high-efficient enzymatic hydrolysis was studied. Research showed that the efficiency of hydrolysate could be improved by ultrasonic treatment. Compared with control group, inhibitory activity of the hydrolysate was increased by 23.96% after the treatment of 600 W for 10 min. The fluorescence intensity of DWGP after ultrasonic treatment was found discovered to be changed. An appropriate ultrasonic treatment can unfold the protein molecule and make the chromogenic groups uncovered, which contribute to the acquirement of the higher-activity inhibitory peptide. The effects of various ultrasonic power and time on the secondary structure of DWGP were quantitatively determined via analysis of the amide Ⅰ changes of infrared spectra using curve fitting method. Content of β-sheet was decreased and β-turn was increased after ultrasonic treatment, which could be the main factor to make the prepared inhibitory peptides high efficient. The results provide a theoretical basis for the mechanism research of enzymatic hydrolysis of ultrasonic treated protein.
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Received: 2010-04-07
Accepted: 2010-08-28
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Corresponding Authors:
MA Hai-le
E-mail: mhl@ujs.edu.cn
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[1] JIA Jun-qiang,MA Hai-le,ZHAO Wei-rui,et al(贾俊强,马海乐,赵伟睿,等). Chinese Journal of Process Engineering(过程工程学报),2009,9: 107. [2] DING Qing-zhi, MA Hai-le,LUO Lin,et al(丁青芝, 马海乐, 骆 林, 等). Chinese Society of Agricultural Engineering(农业工程学报),2009,25(1): 294. [3] HE Rong-hai, MA Hai-le, ZHOU Cun-shan,et al(何荣海,马海乐,周存山,等). Journal of Jiangsu University(Natural Science Edition)(江苏大学学报·自然科学版),2007, 28(1):4. [4] Rajesh Krishnamurthy, Janice A. International Journal of Pharmaceutics,20002,245(1): 23. [5] Marchioni C, Riccardi E, Spinelli S, et al. Ultrasonics,2009,49(6): 569. [6] Oulahala N, Adta I, Mariani C, et al. Food Control,2009, 20(7): 658. [7] Murray B A, Walsh D J, Fitz Gerald R J. Journal of Biochemical and Biophysical Methods,2004,(59): 127. [8] Jens Adler-Nissen,Svend Eriksen,Hans Sejr Olsen. Qual. Plant Plant Foods Hum. Nutr., 1983,32:411. [9] Byler D M, Susi H. Bio-polymers, 1986, 25(3): 469. [10] Oulahala N, Adta I, Mariani C,et al. Food Control, 2009, 20(7): 658. [11] Servagent-Noinvillea S, Revaulta M, Quiquampoixb H,et al. Journal of Colloid and Interface Science,2000, 221(2): 273. [12] ZHANG Yan, CHEN Pei-rong, ZHOU Hai-meng(张 艳,陈培榕,周海梦). Acta Biophysica Sinica(生物物理学报), 1994, 10: 181. [13] Byler D M, Brouillette J N, Susi H. Spectroscopy,1986,1: 29. [14] JIA Jun-qiang,MA Hai-le,WANG Zhen-bin,et al(贾俊强,马海乐,王振斌,等). Journal of the Chinese Cereals and Oils Association(中国粮油学报),2009,24(5):110.
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