光谱学与光谱分析 |
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Study of Tyrosine Online Fluorescence Spectrum under High Pressure |
JIANG Chun-yue1, XIANG Run-qing1*, BAO Cheng-man2,GAI Yun2 |
1. College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, China 2. College of Biomedical Engineering & Instrument Science,Zhejiang University,Hangzhou 310027, China |
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Abstract The effect of pressure on fluorescence of tyrosine (Tyr) and Tyr in different concentration of Cu2+ was investigated. The results showed that Tyr fluorescence intensity was enhanced with increasing pressure in the absence of Cu2+, with the fluorescence intensity increasing by about 9% when the pressure reached 60 MPa. Furthermore, Tyr fluorescence quenched by Cu2+ and the quenching became stronger when the concentration of Cu2+ was higher. The effect of pressure on the fluorescence of Tyr was different under various Cu2+ concentrations. When Cu2+ concentration was lower, the fluorescence intensity increased relatively weakly (under 60 MPa and [Copper ion]/[Tyr]=1, fluorescence intensity increased by 14.4%) and vice versa, the fluorescence intensity increased relatively strongly (under 60 MPa and [Copper ion]/[Tyr]=40, fluorescence intensity increased by 38.4%).
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Received: 2010-07-26
Accepted: 2010-10-18
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Corresponding Authors:
XIANG Run-qing
E-mail: xiangrunqing@126.com
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