| 光谱学与光谱分析 |
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| Spectral Study on the Interaction of [Cu(DPPZ)(L-Ser)]+ Complex with DNA and Its Analytical Application |
| WU Cheng-ke1, DONG Qiu-peng1，2, ZHANG Hong1, XIONG Cen1, WU Xiu-li1, LING Lian-sheng1* |
1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China
2. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China |
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Abstract The characteristics of resonance light scattering (RLS), UV-visible absorption spectra and fluorescence spectra of [Cu(DPPZ)(L-Ser)]+ with DNA were studied and a RLS method for the determination of DNA was established. [Cu(DPPZ)(L-Ser)]+ could assemble on the surface of DNA through intercalation, and enhanced the RLS of DNA in the tris buffer of pH 7.2. The maximum resonance light scattering peak appeared at 400 nm. Under the optimum conditions, the enhanced intensity of RLS was proportional to the concentration of DNA over the range of 0.42～4.2 ng·mL-1, with a detection limit (3σ/k) of 0.29 ng·mL-1. The method was used for the determination of DNA samples with the recoveries between 97.8% and 106%.
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Received: 2010-05-08
Accepted: 2010-08-08
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Corresponding Authors:
LING Lian-sheng
E-mail: cesllsh@mail.sysu.edu.cn
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