| 光谱学与光谱分析 |
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| Study on Saltation of Fluorescence Excitation Spectra of Erythrosine |
| MA Chao-qun1, CHEN Guo-qing1*, WEI Bai-lin1, SHI Yuan-ping1, GU Ling1, GAO Shu-mei1, ZHU Tuo1, 2 |
1. School of Science, Jiangnan University, Wuxi 214122, China
2. School of Energy and Electric, Hehai University, Nanjing 210098, China |
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Abstract The fluorescence excitation spectra and absorption spectra of six kinds of erythrosine solutions with concentrations of 10, 20, 30, 40, 50 and 60 μg·mL-1 were experimentally measured. It was found that the fluorescence excitation peaks are both located at 530 nm significantly when the concentrations of erythrosine solutions are 10 and 20 μg·mL-1. However, the linetype saltation of fluorescence excitation spectrum occurs as the concentration of erythrosine solution is above 30 μg·mL-1. The valley is located at 530 nm and two new peaks appear at both flanks of the valley. Compared with fluorescence excitation spectra, the absorption spectra of erythrosine solutions are without saltation and the peaks are all located at 530 nm. According to calculations and a series of contrast experiments, it was demonstrated that the absorption characteristic of erythrosine and the spectral measurement mode conspire to cause the saltation of fluorescence excitation spectra. The results can provide guidance for further research on physical and chemical properties of erythrosine, and offer help and reference for study on saltation behavior in fluorescence excitation spectra and improvement in spectral measuring mode.
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Received: 2010-07-16
Accepted: 2010-10-22
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Corresponding Authors:
CHEN Guo-qing
E-mail: cgq2098@163.com
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