| 光谱学与光谱分析 |
|
|
|
|
|
| A New Optical Switch Using Upconversion Nanoparticles Conjugated Aptamer |
| SONG Kai1, 2, KONG Xiang-gui1* |
1. Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
|
|
|
|
Abstract As new class of fluorescent biolabels, upconversion nanoparticles (UCNPs) were very attractive for use as labels, compared with these traditional downconversion materials. It can eliminate problems associated with autofluorescence and scattered excitation light under near infrared light (NIR) excitation. In the present work, water-soluble NaYF4∶Yb3+, Er3+ UCNPs were successfully prepared by solvothermal synthesis, using branched polyethylenimine as the surfactants, and a generic design strategy for UCNPs conjugated aptamer based optical switch was presented. In the absence of the target thrombin protein, such a duplex structure brings the UCNPs and TAMRA close proximity of each other, leading to luminescence resonance energy transfer from UCNPs to TAMRA upon near-infrared light irradiation. When target is introduced, the quadruplex conformation of the aptamer should be preferentially stabilized, resulting in the displacement of the TAMRA labelled oligonucleotide conjugate and triggering UCNPs fluorescence intensity to increase and the corresponding TAMRA fluorescence intensity to decrease. Since the aptamer responds to its target molecule by switching on its fluorescent properties, the authors named this an “optical switch”.
|
|
Received: 2010-05-02
Accepted: 2010-08-06
|
|
|
|
Corresponding Authors:
KONG Xiang-gui
E-mail: xgkong14@ciomp.ac.cn
|
|
[1] Ellington A D, Szostak J W. Nature, 1992, 355: 850.
[2] Tereshko V, Skripkin E, Patel D. J. Chem. Biol., 2003, 10(2): 175.
[3] Zimmerman J M, Maher L J. Nucl. Acids Res., 2002, 30(9): 5425.
[4] Wen J D, Gray C W, Gray D M. Biochemistry, 2001, 40(31): 9300.
[5] Navani N K, Li Y F. Curr. Opin. Chem. Biol., 2006, 10(3): 272.
[6] Yang C J, Jockusch S, Vicens M, et al. Proc. Natl. Acad. Sci. U.S.A., 2005, 102(48): 17278.
[7] Song S, Wang L, Li J, et al. Trends Anal. Chem., 2008, 27(2): 108.
[8] Wang M, Hou W, Mi C C, et al. Anal. Chem., 2009, 81 (21): 8783.
[9] SONG Kai, TIAN Li-jin, KONG Xiang-gui, et al(宋 凯，田利金，孔祥贵，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2010, 30(1): 133.
[10] Auzel F. Chem. Rev., 2004, 104(1): 139.
[11] Thomas T P, Myaing M T, Ye J Y, et al. Biophys., 2004, 86(6): 3959.
[12] Feijo J A, Moreno N. Protoplasma, 2004, 223(1): 1.
[13] Boyer J C, Cuccia L A, Capobianco J A. Nano Lett., 2007, 7(3): 847.
[14] Pollnau M, Gamelin D R, Lüthi S R, et al. Phys. Rev. B, 2001, 61(5): 3337.
[15] Chen Z, Chen H, Hu H, et al. J. Am. Chem. Soc., 2008, 130(10): 3023.
[16] Elwell C A, Dreyfus L A. Molecular Microbiology, 2000, 37(4): 952.
[17] Choi J H, Chen K H, Strano M S. J. Am. Chem. Soc., 2006, 128(49): 15584. |
| [1] |
DING Han. Background-Free Development of Latent Fingerprints on Fluorescent
Substrates[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3427-3435. |
| [2] |
FAN Dan-yang1, ZHANG Xue-cheng1, GAO Jun1, WANG Jia-bin2, LÜ Hai-xia1*. Study on Specific Detection of Sulfadimethoxine Based on Aptamer-Modified Up-Conversion Fluorescent Nanomaterial[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3409-3414. |
| [3] |
WANG Chong1, WANG Jing-hua1, 2, LI Dong-dong1, SHE Jiang-bo2. Preparation of Gd3+-Doped LiYF4∶Yb3+/Ho3+ Micro-Crystal and the Application Research in Anti-Counterfeiting[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3581-3587. |
| [4] |
ZHANG Li-gang1, MA Li-hong1*, ZHAO Su-ling2, XU Zheng2, YANG Hai-jun1, LI Chen-pu1, WANG Ke1, LIU Gui-xia1, BAI Yong-qing1, SHEN Wen-mei1. Effect of Reaction Temperature on the Luminescence and Morphology of Na3ScF6∶Yb/Er Nanocrystals[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3068-3072. |
| [5] |
XU Lei, ZHU Lin, ZHANG Chun, YE Chang-qing*, CHEN Shuo-ran, LI Lin, LIANG Zuo-qin, WANG Xiao-mei*. Study on Properties of Azaanthracene Derivatives With Triplet-Triplet Annihilation Upconversion and One-Photon Hot Band Absorption
Upconversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1761-1768. |
| [6] |
LIANG Zuo-qin, YAN Xu, SONG Dong-dong, ZHANG Xiao-bo, ZHANG Jia-xuan, YE Chang-qing, CHEN Shuo-ran, WANG Xiao-mei. The Influence of Substituents in Anthracene Derivatives on the Performance of Triplet-Triplet Annihilation Upconversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 802-807. |
| [7] |
WANG Chong1, MO Jian-ye1，2, LI Dong-dong1, SHE Jiang-bo2, LIU Zhen2. Application and Research of NaYF4∶Yb3+/Eu3+ Upconverting Luminescent Micro-Nano Particles in Anti-Counterfeiting Identification[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1525-1529. |
| [8] |
YE Chang-qing, YU Xue, CHEN Shuo-ran, LIANG Zuo-qin, ZHOU Yu-yang, WANG Xiao-mei*. Study on the Structure/Energy-Level of Palladuim-Porphyrin Sensitizers on the Triplet-Triplet-Annihilation Upconversion Performance[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 71-79. |
| [9] |
CHEN Xiao-bo1, LI Song1, ZHAO Guo-ying2, LONG Jiang-mi1, WANG Shui-feng1, MENG Shao-hua2, WANG Jie-liang1, GUO Jing-hua1, YOU Jia-jia1, MA Yu2, YU Chun-lei3, HU Li-li3. The Silver Surface Plasmon Enhancement for Er3+ Ion Upconversion of 978 and 1 539 nm Laser in Bismuth Glass[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2721-2726. |
| [10] |
CHEN Shuo-ran, HUANG Su-qin, HAN Peng-ju*, YE Chang-qing, SONG Sa-sa, WANG Xiao-mei*. Preparation of 9,10-Diphenylanthracene Derivative and Its Detection for Cu2+ by Up/Down-Conversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3769-3775. |
| [11] |
CHEN Shuo-ran1, ZHENG Dao-yuan1, LIU Teng1, YE Chang-qing1*, SONG Yan-lin2. Ratiometric Fluorescent Temperature Probe Based on Up/Down-Conversion Luminescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3088-3095. |
| [12] |
WU Qi-xiao1, 2, ZHAO Su-ling1, 2*, XU Zheng1, 2, SONG Dan-dan1, 2, QIAO Bo1, 2, ZHANG Jun-jie1, 2, ZUO Peng-fei1, 2. Synthesis and Upconversion Mechanism of NaYF4∶Yb3+,Er3+ Nanocrystal Doped with Different Concentration of Sensitizer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1406-1411. |
| [13] |
ZHANG Yang, SUN Peng, LIU Lu*, WANG De-xing, CHEN Shu-yan*, CHENG Li, SU Li-ping, ZHU Zheng, CHEN Yang. Effects of Li Ions on the Thermal Radiation Induced by NIR Laser in Rare Earth Doped Oxide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2725-2729. |
| [14] |
CHEN Jia1, YE Chang-qing1, ZHU Sai-jiang1, WANG Xiao-mei1,2*, TAO Xu-tang2. Synthesis of 9,10-Diheterocyclicanthracenes and Performance Correlations in Triplet-Triplet Annihilation Upconversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 715-721. |
| [15] |
ZHANG Li-gang1，2, ZHAO Su-ling1*, XU Zheng1, ZHU Wei1, BAI Yong-qing2, QU Jiao2, FAN Hong2. The Controllable Synthesis and Luminescent Properties of ScF3, NaScF4, (NH4)2NaScF6 Nanaocrysals[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 401-406. |
|
|
|
|
