| 光谱学与光谱分析 |
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| Study on the Content Determination of Total Flavonoids in Olea europaea L. Leaves |
| ZHENG Yuan-yuan1, 2, LI Chen2, 3, FENG Shi-lan1, HUANG Xin-yi2, LIU Yong-feng2, 4, CHEN Xiao-fen2, 4, DI Duo-long1，2* |
1. Lanzhou University Pharmaceutical College, Lanzhou 730000, China
2. Key Laboratory of Chemistry of Northwestern Plant Resources & Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
3. Instrumental Analysis & Research Center, School of Applied Physics & Material Engineering, Wuyi University, Jiangmen 529020, China
4. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract A quantitative determination method of the total flavonoids in Olea europaea L. leaves using rutin as a standard substance was developed. Two coloration systems including Al(NO3)3-NaNO2-NaOH and AlCl3 were both used. The latter was selected as the optimal determination method by comparing with the results. The main interference of the matrix was discussed. The result showed that the AlCl3 coloration method is simple, accurate and reproducible with good linear relationship, and it can be looked as a reference method for the determination of total flavonoids in herbal materials or natural products.
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Received: 2010-05-10
Accepted: 2010-08-20
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Corresponding Authors:
DI Duo-long
E-mail: didl@licp.cas.cn
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[1] WANG Xiaofei, LI Chen, SHI Yanping, et al. J. Asian Nat. Prod. Res., 2009, 11: 940.
[2] Manuel B, Aranzazu G, Pedro G, et al. J. Agric. Food Chem., 1999, 47: 3535.
[3] Aurelia N S, Carla D’ Orazio, Vanessa R, et al. Int. J. Antimicrob Ag, 2009, 33: 461.
[4] Raffaella B, Maurizio P, Stefano T, et al. J. Agric. Food Chem., 2002, 50: 4934.
[5] Heimler D, ieroni A, Tattini M, et al. Chromatographia, 1992, 33(728): 369.
[6] Jeffrey B H, Christine A W. Phytochemistry, 2000, 55: 481.
[7] Rong Tsao, Zeyuan Deng. J. Chromatogr. B, 2004, 812: 85.
[8] Josep V, Silvia M, Pilar M, et al. J. Chromatogr. A, 2009, 1216: 7143.
[9] Aline M, Charles E L, Marco R. Food Chem., 2005, 91: 571.
[10] Cyril S, Béatrice B, Riad E, et al. J. Agric. Food Chem., 2001, 49: 618.
[11] LIU Dong-ping, ZHOU Ya-qiu, WANG Xian-rong(刘东平, 周亚球, 王先荣). Lishizhen Medicine and Materia Medica Research(时珍国医国药), 2007, 18 (3): 614.
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