| 光谱学与光谱分析 |
|
|
|
|
|
| Spectroscopic Study on the Conformational Change of Silk Fibroin in Methanol-Water Mixtures |
| MA Lin，HE Wei-ren，HUANG Ai-min，LI Li-shuo，TONG Zhang-fa, WEI Qiao-na，HUANG Zi-lun |
| College of Chemistry and Chemical Engineering，Guangxi University，Nanning 530004，China |
|
|
|
|
Abstract A combinational study of circular dichroism，intrinsic fluorescence of protein and exogenous fluorescence probe of ANS was made to investigate the conformational change of silk fibroin in methanol-water mixtures as well as the mechanism.The spectral results showed that small hydrophobic regions were formed in silk fibroin in methanol-water mixtures at the concentration lower than 30% (V/V) via hydrophobic interaction，which was decreased at higher methanol content due to a structural transition of silk fibroin from random coil to β-sheet.The conformational change of silk fibroin was found to be of a close relationship with the microstructure of the solvent and to be determined by the interaction between the peptide unit of silk fibroin and the cluster of the mixed solvent.Methanol-water mixture at low concentration had little effect on the solvation of the peptide unit and the conformation of silk fibroin，as a consequence of the fact that the inherent water structure was conserved.The transition from the tetrahedral-like water structure to the chain-like methanol structure，due to the increasing concentration of methanol，induced the conformational change of silk fibroin to eliminate the contact of peptide unit with the solvent molecular.
|
|
Received: 2009-12-16
Accepted: 2010-03-22
|
|
|
|
Corresponding Authors:
MA Lin
E-mail: malinzju@gxu.edu.com
|
|
[1] Zhou P，Xie X，Knight D P，et al. Biochemistry，2004，43：11302.
[2] Nakazawa Y，Asakura T. J. Am. Chem. Soc.，2003，125：7230.
[3] Tanaka C，Takahashi R，Asano A，et al. Macromolecules，2008，9：796.
[4] Kino R，Ikoma T，Yunoki S，et al. J. Biosci. Bioeng.，2007，103：514.
[5] Wenk E，Wandrey A J，Merkle H P，et al. J. Control. Release，2008，132：26.
[6] Haouz A，Mohsn S E，Zentz C，et al. Eur. J. Biochem.，1999，264：250.
[7] Brahms S，Brahms J，Spach G，et al. Proc. Natl. Acad. Sci. USA，1977，74：3208.
[8] Johnson W C. Annual Rev. Biophys. Biophys. Chem.，1988，17：145.
[9] Lakowicz J R. Principles of Fluorescence Spectroscopy. 2nd ed. New York：Plenum Press，1999. 185.
[10] Schay G，Smeller L，Tsuneshige A，et al. J. Biol. Chem.，2006，281：25972.
[11] Burstein E A，Vedenkina N S，Ivkova M N. Photochem. Photobiol.，1973，18：263.
[12] Yang Y H，Shao Z Z，Chen X，et al. Biomacromolecules，2004，5(3)：773.
[13] Gasymov O K，Glasgow B J. Biochimica et Biophysica Acta，2007，1774：403.
[14] Kosower E M. Annu. Rev. Phys. Chem.，1986，37：127.
[15] Lee J，Robinson G W. J. Am. Chem. Soc.，1985，107：6153.
[16] Nozaki Y，Tanford C. J. Biol. Chem.，1971，246：2211.
[17] Parodi R M，Bianchi E，Ciferri A. J. Biol. Chem.，1973，248：4047.
[18] Wakisaka A，Komatsu S，Usui Y. J. Mol. Liq.，2001，90：175.
[19] Yoshida K, Kitajo A, Yamaguchi T. J. Mol. Liq., 2006, 125: 158.
[20] Ma L，Wang X，Xu L，et al.Chin.J.Chem.，2008，10：1793. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
