| 光谱学与光谱分析 |
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| The Simultaneous Measurements of Time-Dependent Laser-Induced Fluorescence Intensity of Hematoporphyrin Monomethyl Ether in Animal Tissues with Rheumatoid Arthritis |
| YU Chang-qing1，3, HUANG Nai-yan2, ZHAO Hai-ying1, DOU Xiao-ming1, LI Jia-ze3 |
1. Molecular Photonics Laboratory, Physics Department, Shanghai Jiaotong University, Shanghai 200030, China
2. Department of Laser Medicine, People's Liberation Army General Hospital, Beijing 100853, China
3. Photonics Laboratory, Department of Optical Engineering, Beijing Institute of Technology, Beijing 100081, China |
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Abstract Since the selectivity of photodynamic therapy (PDT) depends on the distribution of a photosensitizer in a tissue during the treatment, an investigation of drug distribution is a key step for performing PDT effectively. The distribution of photosensitizer absorbed in tissues is adjusted by the animal body system, so an apparatus that can measure the fluorescence intensity of photosensitizer in different tissues of the same body simultaneously is in demand. To achieve precise estimate of tissue selectivity of the photosensitizer, a spatially separated three-channel laser-induced fluorescence (LIF) detection system was set up and employed in the present study to measure the fluorescence intensity of Hematoporphyrin Monomethyl Ether (HMME) in different tissues of the same body simultaneously. The time-dependent variations in the concentrations of HMME within the skin, cartilage, normal synovium and inflammatory synovium of rabbit were monitored in vivo. The results obtained showed that the synovium has higher absorptivity of HMME than the skin and cartilage. The difference is distinct from the very beginning of injection. Although the quantity of HMME absorbed in the inflammatory synovium is not very high in the first 20 min, it is still 6 times higher than that in the skin and cartilage. In addition, the absorptivity of HMME is much stronger for the inflammatory synovium than that for the normal synovium. If the laser beam irradiates outside the joint for the rheumatoid arthritis, tissues around the inflammatory synovium have less HMME, thereby causing weak PDT effect. This would help reduce the side effect of PDT. Thus we suggest that for PDT treated rheumatoid arthritis，taｋing the first 20 min after the injection for outside-the-joint eｘcitation employing HMME maybe a good choice.
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Received: 2002-10-11
Accepted: 2003-02-27
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Corresponding Authors:
YU Chang-qing
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| Cite this article: |
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YU Chang-qing,HUANG Nai-yan,ZHAO Hai-ying, et al. The Simultaneous Measurements of Time-Dependent Laser-Induced Fluorescence Intensity of Hematoporphyrin Monomethyl Ether in Animal Tissues with Rheumatoid Arthritis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 138-141.
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http://www.gpxygpfx.com/EN/Y2004/V24/I02/138 |
[1] Firestein G S, Zcaifler N J. J. Arthritis Rheum., 1990，33: 768.
[2] Okunaka T, Kato H. Reviews in Contemporary Pharmacotherapy，1999，10(1): 59.
[3] Ochsner M. Photodynamic Therapy，199７，47(11): 1185.
[4] GU Ying，LI Jun-heng，GUO Zhong-he et al(顾 瑛，李俊亨，郭中和等). Beijing Medicine(北京医学)，1991，13(5)：317.
[5] Kirby B. British J. of Dermatology, 2001，144: 37.
[6] Lipson R. Baldes E. J. Thorac. Cardiovasc. Surg.，1961，42：623.
[7] Trauner K B, Gandour-Edwards R, Bamberg M et al. Photochemistry and Photobiology, 1998，67: 133.
[8] Trauner K B, Gandour-Edwards R, Bamberg M et al. Lasers in Surgery and Medicine, 1998，22: 147.
[9] Hendrich C, Huttmann G, Vispo-Seara J L et al. Knee Surgery Sports Traumatology Arthroscopy, 2000，8(3): 190.
[10] CHEN Wen-hui，YU Jian-ｘin，YAO Jian-zhong et al(陈文晖，余建鑫，姚建忠等). Chin． J． Laser Med． Surg.(中国激光医学杂志)，2000，9(2)：105.
[11] GU Ying，LIU Fan-guang，FU Ｑiu-tao et al(顾 瑛，刘凡光，富秋涛等). Chin． J． Laser Med． Surg.(中国激光医学杂志)，2000，9(1)：1.
[12] FU Ｑiu-tao，GU Ying，LIU Fan-guang et al(富秋涛，顾 瑛，刘凡光等). Acta Laser Biology Sinica(激光生物学报)，2000，9(2)：13７.
[13] LIU Fan-guang，GU Ying，GUAN Cheng-yu et al(刘凡光，顾 瑛，关澄宇等). Acta Laser Biology Sinica(激光生物学报)，2000，9(3)：206.
[14] DENG Xiao-hu，GU Ying，HUANG Feng et al(邓小虎，顾 瑛，黄 烽等). Chin． J． Laser Med． Surg.(中国激光医学杂志)，2001，10(4)：218.
[15] Goodfellow R M, Williams A S, Levin J L et al. Clin. Exp. Immunol.，2000，119: 210.
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