光谱学与光谱分析 |
|
|
|
|
|
Characterization of Reaction Kinetics between Ligand and Receptor on the Solid and Liquid Interface Based on Optical Waveguide Lightmode Spectroscopy |
WU Zhong-wei, LIU Quan-jun, WU Ling-wei, XIE Xiao, LU Zu-hong* |
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China |
|
|
Abstract The present paper describes the use of optical waveguide lightmode spectroscopy (OWLS) for study of the binding interaction of the vascular endothelial growth factor (VGEF) with VEGF receptor 2 (VEGFR2). VEGF were immobilized in the surface of an 3-amino 3-propyltriethoxy silane(APTES) modified sensor chip. The solutions with different concentration of VEGFR2 were injected to the system to investigate the kinetic character with OWLS on the solid and liquid interface. The receptor binding and dissociation on the interface, quantified by association and dissociation rate coefficients ka and kd, were determined by the OWLS experiments. The ka and kd is 6.86×105 L·mol-1·s-1 and 1.15×10-3 s-1, respectively. The results show that OWLS method could meet the requirement of kinetic determination of ligand-receptor interaction in applications for related fundamental research and pharmaceutical development.
|
Received: 2009-10-28
Accepted: 2010-01-29
|
|
Corresponding Authors:
LU Zu-hong
E-mail: zhlu@seu.edu.cn
|
|
[1] Li S R, Peck-Radosavljevic M, Koller E. International Journal of Cancer, 2001, 91(6): 789. [2] von Tiedemann B, Bilitewski U. Biosensors & Bioelectronics, 2002, 17(11-12): 983. [3] CHAO Ke-fu, ZHANG You-lin, KONG Xiang-gui, et al(朝克夫,张友林,孔祥贵,等). Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2008, 28(7): 1603. [4] Huang X M, Gottstein C, Brekken R A. Biochemical and Biophysical Research Communications, 1998, 252(3): 643. [5] Ramsden J J. Quarterly Reviews of Biophysics, 1994, 27(1): 41. [6] Lindner R, Ralston G. Biophysical Chemistry, 1995, 57(1): 15. [7] Chiu M L, Viollier P H, Katoh T. Biochemistry, 2001, 40(43): 12950. [8] WU Zhong-wei, LIU Qian-jun, WU Ling-wei(吴中伟,刘全俊,吴凌伟). Acta Optica Sinica(光学学报),2007, 27(3): 521. [9] Ferrara N, Henzel W J. Biochemical and Biophysical Research Communications, 1989, 161(2): 851. [10] Mac Gabhann F, Popel A S. American Journal of Physiology-Heart and Circulatory Physiology, 2004, 286(1): H153. [11] Sho E, Komatsu M, Sho M. Experimental and Molecular Pathology, 2003, 75(1): 1. [12] Voros J, Ramsden J J, Csucs G. Biomaterials, 2002, 23(17): 3699. [13] Alfthan K. Biosensors & Bioelectronics, 1998, 13(6): 653. [14] Karlsson R. Analytical Biochemistry, 1994, 221(1): 142. [15] Calonder C, Van Tassel P R. Langmuir, 2001, 17(14): 4392.
|
[1] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
[2] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
[3] |
ZHU Shao-hao1, SUN Xue-ping1, TAN Jing-ying1, YANG Dong-xu1, WANG Hai-xia2*, WANG Xiu-zhong1*. Study on a New Sensing Method of Colorimetric and Fluorescence Dual Modes for Pesticide Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2785-2791. |
[4] |
LUO Dong-jie, WANG Meng, ZHANG Xiao-shuan, XIAO Xin-qing*. Vis/NIR Based Spectral Sensing for SSC of Table Grapes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2146-2152. |
[5] |
ZHANG Hai-yang, ZHANG Yao*, TIAN Ze-zhong, WU Jiang-mei, LI Min-zan, LIU Kai-di. Extraction of Planting Structure of Winter Wheat Using GBDT and Google Earth Engine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 597-607. |
[6] |
ZHU Xiao-ming1, 2, 3, BAI Xian-yong1, 2, 3*, LIN Jia-ben1, 2, DUAN Wei1, 2, ZHANG Zhi-yong1, 2, FENG Zhi-wei1, 2, DENG Yuan-yong1, 2, YANG Xiao1, 2, HUANG Wei1, 2, 3, HU Xing1, 2, 3. Design and Realization of High-Speed Acquisition System for Two Dimensional Fourier Transform Solar Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3842-3850. |
[7] |
WANG Wei, LI Yong-yu*, PENG Yan-kun, YANG Yan-ming, YAN Shuai, MA Shao-jin. Design and Experiment of a Handheld Multi-Channel Discrete Spectrum Detection Device for Potato Processing Quality[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3889-3895. |
[8] |
JIN Wang-jun1, 2, LI Yan1, 2, ZHAO Yue3, MEI Sheng-hua1*. In Situ Raman Study and Kinetic Analysis of Hydrothermal Liquefaction of Glycine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3448-3453. |
[9] |
FAN Yi-guang1, 3, 5, FENG Hai-kuan1, 2, 3*, LIU Yang1, 3, 4, BIAN Ming-bo1, 3, ZHAO Yu1, 3, YANG Gui-jun1, 3, QIAN Jian-guo5. Estimation of Potato Plant Nitrogen Content Using UAV Multi-Source Sensor Information[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3217-3225. |
[10] |
DENG Ya-li1, LI Mei2, WANG Ming2*, HAO Hui1*, XIA Wei1. Surface Plasmon Resonance Gas Sensor Based on Silver/Titanium Dioxide Composite Film[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 743-748. |
[11] |
WANG Xin-hui1, 2, GONG Cai-lan1, 2*, HU Yong1, 2, LI Lan1, 2, HE Zhi-jie1, 2. Spectral Feature Construction and Sensitivity Analysis of Water Quality Parameters Remote Sensing Inversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1880-1885. |
[12] |
YANG Jun1,QI Li-mei1*,WU Li-qin2,LAN Feng3,LAN Chu-wen4,5,TAO Xiang1,LIU Zi-yu1. Research Progress of Terahertz Metamaterial Biosensors[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1669-1677. |
[13] |
JIANG Li-ying, XU Xiao-ping, QIN Zi-rui, ZHANG Pei, MENG Xiao-long, REN Lin-jiao*, WANG Wei*. A Fluorescence Aptasensor for Detecting Silver Ion Concentration in Aqueous Environment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1066-1071. |
[14] |
CHEN Yu1, WEI Yong-ming1, WANG Qin-jun1,2*, LI Lin3, LEI Shao-hua4, LU Chun-yan5. Effects of Different Spectral Resolutions on Modeling Soil Components[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 865-870. |
[15] |
LIU Xue-mei, WANG Xiao-lin, QIU Zeng-feng, WANG Ya-dong, ZHANG Bin, XU Chao*, YIN Hong-zong*. Surface Plasmon Resonance Sensing Technology is Applied to Small Molecule Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 511-516. |
|
|
|
|