| 光谱学与光谱分析 |
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| Study of Microstructure of Pectin and Whey Protein Isolate Mixtures under Incompatible Conditions Using Dynamic Light Scattering and Transmission Electron Microscopy |
| LENG Xiao-jing1, CHAI Zhi1, REN Fa-zheng1, ZHANG Lu-da2, Turgeon S L3 |
1. Laboratory of Functional Dairy, Ministry of Education and Beijing City, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2. College of Science, China Agricultural University, Beijing 100094, China
3. Centre de Recherche en Science et Technologie du Lait (STELA), Faculté des Sciences de l’Agriculture et de l’Alimentation. Université Laval, Pavillon Paul-Comtois, Sainte-Foy, Québec, Canada G1K 7P4 |
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Abstract The microstructure of the pectin/whey protein isolate mixtures under incompatible conditions was investigated using dynamic light scattering spectroscopy, transmission electron microscopy and shear-viscosity model. Under the condition of 90 ℃ and pH 7.4, the presence of negatively charged pectin could induce depletion aggregation in a 5% protein solution, and promote phase separation; precisely, when the mass ratio of pectin/whey protein isolate was lower than 0.08, the hydrodynamic size of the aggregates was less than 300 nm, and the system showed Newtonian properties; when the mass ratio was higher than 0.08, the viscosity of the solution increased rapidly, the shear thinning properties became obvious and the size of the aggregates was close to 700 nm.
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Received: 2009-11-02
Accepted: 2010-02-06
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Corresponding Authors:
LENG Xiao-jing
E-mail: xiaojing.leng@gmail.com
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