光谱学与光谱分析 |
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Study on the Effect of GDG on Secondary Structure of PLasminogen and Plasminogen Activators by Circular Dichroism |
HAO Fei,WU Wen-hui,QU Jia-hua,BAO Bin* |
College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China |
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Abstract To investigate the effect of GDG on the secondary structure of plasminogen and plasminogen activators, circular dichroism and SDS-PAGD were applied. The results show that the secondary structures of prourokinase and streptokinase were changed by GDG. The amount of α-helix, β-sheet, β-turn and random coil of fibrinolytic factors relates with the different concentrations of GDG in the study. GDG has no effects on the secondary structure of plasmin and plasminogen. GDG enhancing the interactivation of plasminogen and prourokinase was indicated by SDS-PAGE. It was found that GDG significantly affected the activity of prourokinase and streptokinase, and increased intrinsic fluorescence of prourokinase.
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Received: 2009-10-08
Accepted: 2010-01-09
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Corresponding Authors:
BAO Bin
E-mail: bbao@shou.edu.cn
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