| 光谱学与光谱分析 |
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| UV-Vis Absorption Spectral Characteristics of Tobacco PeroxidaseⅠ from Nicotiana Tabacum |
| XIA Bing-le, LI Min-li, LIU Qing-liang, ZHU Xiao-lan |
| Department of Chemistry, University of Science and Technology of China, Hefei 230026，China |
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Abstract The ultraviolet／visible spectra of TOP Ⅰ were studied. It was proved that TOP Ⅰ is an acid enzyme containing hemochrome as an agon. When the pH reduced, the Soret absorption in UV-Vis region eｘhibited a blue shift，when pH increased it eｘhibited a red shift. The result of the influence of carbamide, the denaturant, on the spectra showed that TOP Ⅰ may have a unfolded structural change in the solution, which made the peptide chain completely extend. After adding Fe(Ⅲ), Fe(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Co(Ⅱ), Ni(Ⅱ) and Sn(Ⅱ) to the apo-TOP Ⅰ, the UV-Vis spectra changed except for Fe(Ⅲ), which almost did not change at all. The strongest characteristic absorption peak of the Soret showed a blue shift to different extent and it became weak gradually. The situation of α-strip and β-strip remained unchanged while the relative strength decreased.
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Received: 2002-09-02
Accepted: 2003-02-26
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Corresponding Authors:
XIA Bing-le
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| Cite this article: |
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XIA Bing-le,LI Min-li,LIU Qing-liang, et al. UV-Vis Absorption Spectral Characteristics of Tobacco PeroxidaseⅠ from Nicotiana Tabacum [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 830-833.
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http://www.gpxygpfx.com/EN/Y2004/V24/I07/830 |
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