正交偏振多光谱成像术应用于活体微循环观察研究

doi:10.3964/j.issn.1000-0593(2010)07-1886-04

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Abstract A small, simple intravital microcirculation multi-spectral imaging system was constructed. Orthogonal polarization spectral (OPS) imaging and liquid crystal tunable filter (LCTF) were used for intravital microcirculation multi-spectral imaging. LCTF has excellent imaging quality and low-power loss, without moving parts and image shift. It can provide continuously, rapid and random-access selection of any wavelength in the visible to near-infrared range. An ear of nude mouse was adopted as experiment specimen. The multi-spectral images of ear microvessel of the nude mouse were acquired by this imaging system in two ways (detection polarization angle 90° and 0°). Compared with detection polarization angle 0° way the images acquired by orthogonal polarization spectral imaging way(detection polarization angle 90°) had better contrast and more details. It is concluded that the experiment has proved that the orthogonal polarization multi-spectral technique can enhance the effect of observation of intravital microcirculation.

Key words：OPS imaging;Liquid crystal tunable filter;Microcirculation;Multi-spectral imaging

Received: 2009-09-22 Accepted: 2009-12-26

ZTFLH:

TN247

Corresponding Authors: LEI Jun-feng E-mail: junflei@126.com

Cite this article:

XU Qian,LEI Jun-feng,ZENG Li-bo. Observation of and Research on Intravital Microcirculation Use of Orthogonal Polarization Multi-Spectral Technique [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(07): 1886-1889.

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2010)07-1886-04 OR https://www.gpxygpfx.com/EN/Y2010/V30/I07/1886

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