光谱学与光谱分析 |
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Study on the Effect of Aluminum (Ⅲ) on NADH in the Presence of NH4Ac in Aqueous Solution |
YANG Xiao-di1, 2, MIAO Qiang1, QIAN Sheng-li1, BI Shu-ping1* |
1. State Key Laboratory of Coordination Chemistry, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China 2. College of Chemistry and Environmental Science, Department of Environmental Science, Nanjing Normal University, Nanjing 210097, China |
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Abstract This paper studied the interactions of Al (Ⅲ) and dihydronicotinamide adenine dinucleotide (NADH) in nearly neutral aqueous solutions (pH 6.5) by means of UV-Vis and 1H, 13C-NMR techniques. The results suggested that Al (Ⅲ) interacts with NADH to form Al-NADH complexes by occupying the binding sites of phosphate oxygen atoms O1N and O1A and ribose ring hydroxyl groups, which are the potential recognition sites for substrates, coenzyme and enzyme. In the presence of NH4Ac salt buffer and with Al (Ⅲ) salt solution, NADH will be marked with structural changes at the nicotinamide moiety in contrast with almost no structural changes in Tris-HCl buffer solution with Al (Ⅲ) salt.
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Received: 1900-01-01
Accepted: 1900-01-01
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Corresponding Authors:
BI Shu-ping
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Cite this article: |
YANG Xiao-di,MIAO Qiang,QIAN Sheng-li, et al. Study on the Effect of Aluminum (Ⅲ) on NADH in the Presence of NH4Ac in Aqueous Solution [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(01): 79-82.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I01/79 |
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