| 光谱学与光谱分析 |
|
|
|
|
|
| The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion |
| XIA Bing-le1, PENG Dun-geng1, LI Min-li1, XIAO Hong2, WANG Kai-bao3 |
1. Department of Chemistry, USTC，Hefei 230026, China
2. Zhumadian Cigarette Factory，Zhumadian 463000, China
3. Wuhu Cigarette Factory，Wuhu 241000, China |
|
|
|
|
Abstract Comparing the fluorescence spectra of tobacco peroxidaseⅠ(TOPⅠ) solution and the solutions titrated by iodine anions，the number of binding locus and the binding constant of iodine anion to TOPⅠ were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOPⅠ molecule were discussed.
|
|
Received: 2003-09-23
Accepted: 2004-02-06
|
|
|
|
Corresponding Authors:
XIA Bing-le
|
|
| Cite this article: |
|
XIA Bing-le,PENG Dun-geng,LI Min-li, et al. The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(02): 239-241.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2005/V25/I02/239 |
[1] XIA Bing-le，PENG Dun-geng，LIU Qing-liang(夏炳乐，彭敦耕，刘清亮). Journal of Chemistry on Internet(国际网上化学学报)，2003，5(1)：http:／／www.chemistrymag.org／cji／2003／051001pc.htm.
[2] XIA Bing-le, LIU Qing-liang，XU Xiao-long(夏炳乐，刘清亮，徐小龙). Journal of University of Science and Technology of China(中国科学技术大学学报)，2003，33(1)：107.
[3] WANG Shou-ye，XU Xiao-long，LIU Qing-liang(王守业，徐小龙，刘清亮). Progress in Chemistry(化学进展)，2001，13(4)：257.
[4] XIA Bing-le, LIU Qing-liang，LI Min-li(夏炳乐，刘清亮，李敏莉). Journal of University of Science and Technology of China(中国科学技术大学学报). 2002, 32(5)：601.
[5] TAO Wei-sun, LI Wei, JING Yong-ming(陶慰孙，李 惟，姜涌明). The Molecular Basis of Protein(蛋白质分子基础). Beijing: Higher Ｅducation Ｐress(北京：高等教育出版社)，1995.
[6] XIA Bing-le(夏炳乐). The Studies on the Purification and Spectroscopic Characterization of Tobacco Peroxidase from <i>Nicotiana Tobaccum</i>(烟草过氧化物酶的分离纯化、结构及其光谱特性的研究). Doctor's Graduation Thesis(博士毕业论文). USTC(中国科学技术大学).
[7] Lakowicz J R. Principles of Fluorescence Spectroscopy. New York: Plenum Press, 1983, 257.
[8] ZHA Dan-ming，LI Shu-ting，YANG Yong-fei, TU Chu-qiao, LIANG Hong(查丹明，李舒婷，杨勇飞，涂楚桥，梁 宏). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999，19(6)：788.
[9] YANG Pin et al(杨 频等). The Principle of Inorganic Biochemistry(生物无机化学原理). Beijing: Science Press(北京：科学出版社), 2002.
[10] Lakavicz J R, Weber G. Biochemistry, 1973, 12: 4161.
[11] LU Ji-xin(卢继新). Acta of Chimica Sinica(化学学报)，1997，55：915.
|
| [1] |
YE Zi-yi, LIU Shuang, ZHANG Xin-feng*. Screening of DNA Dyes for Colorimetric Sensing Via Rapidly Inducing Gold Nanoparticles Aggregation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2805-2810. |
| [2] |
ZHANG Jing1, GAO Xuan1, 2, JIN Liang1, WANG Hong-hui1, ZHOU Xi-ping1. Comparisons and Applications of Functional Equations for the Calculation of the Protein-Ligand Binding Constant Based on Fluorescence Spectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3494-3498. |
| [3] |
WANG Huan, GOU Xing-xing, PU Xiao-hua*, WANG Jiao, HU Xiao-bing, LI Zong-xiao. Exploration of the Interaction Mechanism between Doxorubicin Hydrochloride and DNA by Spectroscopic Techniques and Isothermal Titration Calorimetry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 540-545. |
| [4] |
LI Yan, CHANG Fen-fen, GAO Huan-yuan, CAO Qing, JIN Li-e*. Effects of Glycerol on the Spectral Properties of Sodium Caseinate[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(01): 157-161. |
| [5] |
ZHANG Li-na, CHEN Xin, XIA Yang, WU Dan, YU Jing-hua, DU Bin, WEI Qin. Study on Interaction Mechanism between Meso-Tetra-(4-Hydroxyphenyl)-Zn Porphyrin and Bovine Serum Albumin by Fluorescence Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(03): 773-776. |
| [6] |
YAN Mei,CHEN Xin,SUN Shu-ting,MA Hong-min,DU Bin,WEI Qin*. Study on Binding Mechanism of Meso-tetra-(3,5-Dibromo-4-Hydroxyphenyl) Porphyrin with Protein by Fluorescence Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1322-1326. |
| [7] |
HUANG Yan1,3,XU Guo-xing2*,PENG Yi-ru4*,WENG Jia-bao4,5. Study on the Interaction of Tetra-Sulfonated Phathalocyanine Cobalt with Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 407-411. |
| [8] |
LI Wen-juan1,ZHANG Yong2*,YAN Xin-liang2. Studies on the Interaction of Hemoporphyrin Metalporphyrin and Levo Dopa[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1611-1614. |
| [9] |
CHEN Kui-zhi1,PENG Yi-ru1*,LIN Wei1,CAO Yu-hong1,XU Guo-xing2,WENG Jia-bao<sup>1，3 . Synthesis and Characterization of Octa-Carboxylic Phthalocyanine Aluminum and Its Interaction with Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 84-87. |
| [10] |
LI Yan-ping, LI Lai-sheng*,HUANG Zhi-bing, LIU Xu, GE Xiao-hui. Study on the Interaction between p-Octacarboxyphenylazocalix[8]arene and Norfloxacin by Fluorescence Spectrometry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 108-112. |
| [11] |
LIU Hui-jun1，ZHAN Xiu-ming1，LI Ke-bin2，LIU Wei-ping1* . Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 463-466. |
|
|
|
|
