| 光谱学与光谱分析 |
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| Direct Determination of Ammonia in Plasma with Indophenol Method |
| DENG Jian1, XU Jin-sheng2, FAN Hua1, YUAN Ya-li1, LIU Chuan-xiang1 |
1. College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China
2. Department of Chemistry, Hengyang Normal College, Hengyang 421008, China |
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Abstract A new method for direct determination of ammonia in plasmas with indophenol reagent is described. It is shown that using sodium citrate as anticoagulant in phosphate buffer within pH 10.5-11.2, indophenol reagent reacts sensitively with ammonia in the plasmas to form a stable blue compound with a maximum absorption at 630 nm with low interference. The apparent molor absorptivity is 2.48×104 L·mol-1·cm-1，and the linear range 0-1 500 μmol·L-1 (r=0.999 9) with a detection limit of 1.7 μmol·L-1. The relative standard deviation is 1.13%(n=11) and the recoveries are in the range of 93.6%-101.6% with an average recovery of 97.0%. The proposed method has been applied to detect ammonia in the plasmas from 43 healthy persons and 97 patients who suffered from liver diseases with satisfactory results.
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Received: 2003-09-06
Accepted: 2003-12-16
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Corresponding Authors:
DENG Jian
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| Cite this article: |
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DENG Jian,XU Jin-sheng,FAN Hua, et al. Direct Determination of Ammonia in Plasma with Indophenol Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 436-438.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I03/436 |
[1] Tangerman A, Huizenga J R, Stubbe-Dijk A S et al. J. Hepatol., 1991, 13(suppl. 2): 175.
[2] Buttery J E, Ratnaike R N, Chamberlain B R. J. Chin. Chem. Biochem.，1982, 20: 447.
[3] LIU Hong, SUN Jia-qiang, GAO Yan-qing(刘 宏，孙加强，高燕青). Chin. J. Med. Lab. Sci.(中华医学检验杂志)，1996, 19(4): 223.
[4] LI Yong, ZHUANG Yi-yi(李 勇，庄一义). Chin. J. Med. Lab. Sci.(中华医学检验杂志)，1997, 20(1): 21.
[5] Ministry of Health in People's Republic of China(中华人民共和国卫生部医政司). National Regulation for Clinic(Second Edition)(全国临床检验操作规程). Nanjing: Dongnan University Press(南京: 东南大学出版社), 1997. 223.
[6] HUANG Kai-quan, QIAN Zhong, LEI Li et al(黄开泉，钱 忠，雷 黎等). Shanghai J. Med. Lab. Sci.(上海医学检验杂志)，1996, 11(3): 190.
[7] Horn D B, Squire C R. Clin. Chem. Acta，1966, 14(1): 185.
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