光谱学与光谱分析 |
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Research on the Mechanism of Fluorescence Quenching of HRP by NaN3 |
XIA Bing-le, PENG Dun-geng, NIU Hai-xia, LIU Qing-liang |
University of Science and Technology of China, Chemistry Department, Hefei 230026, China |
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Abstract The fluorescence and synchronous fluorescence spectra were employed to study the binding between sodium azide(NaN3) and horseradish peroxidase (HRP), which was affected by the molecular conformation and the microenvironment of the fluorescence residues. The mechanism of the fluorescence quenching of HRP by NaN3 was discussed and the binding constant and the number of binding sites of non-covalent bond between them were calculated.
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Received: 2003-09-10
Accepted: 2004-01-16
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Cite this article: |
XIA Bing-le,PENG Dun-geng,NIU Hai-xia, et al. Research on the Mechanism of Fluorescence Quenching of HRP by NaN3 [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 412-415.
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https://www.gpxygpfx.com/EN/Y2005/V25/I03/412 |
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