| 光谱学与光谱分析 |
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| Determination of Ascorbic Acid by Indirect Fluorimetry |
| TONG Chang-lun, XIANG Guang-hong, LIU Wei-ping |
| Institute of Environmental Science, Zhejiang University, Hangzhou 310029, China |
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Abstract Experiments indicated that cerium(Ⅳ) ion which could not emit fluorescence was deoxidized by ascorbic acid to cerium(Ⅲ) ion which could emit its characteristic fluorescence in water solution, while sodium hexametaphosphate added could greatly enhance the fluorescence intensity of the system. From this, an indirect sensitive method for determining ascorbic acid was developed. The fluorescence intensity of the system was measured in a 1 cm quartz cell with the excitation and emission wavelengths of 303 and 340 nm, respectively. The results showed that the fluorescence intensity of the system presented a linear relationship with the concentration of ascorbic acid in the range of 1.0×10-7-8.0×10-6 mol·L-1, the correlation coefficient r was 0.999 7, and the detection limit(S/N=3) was 1.6×10-8 mol·L-1. The presented method was used to determine ascorbic acid sample and vitamin C tablet, and the results were satisfactory.
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Received: 2004-01-13
Accepted: 2004-05-13
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Corresponding Authors:
TONG Chang-lun
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| Cite this article: |
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TONG Chang-lun,XIANG Guang-hong,LIU Wei-ping. Determination of Ascorbic Acid by Indirect Fluorimetry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 598-600.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I04/598 |
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