光谱学与光谱分析 |
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Determination of Serum Copper and Zinc in Different Chemical Forms by Graphite Furnace Atomic Absorption Spectrometry with Ethanol-EDTA Precipitation Method |
ZHANG Yuan, LIN Zhe-xuan,LI Hui,PENG Lin, LUO Hong-jun,LI Wei-qiu,LUO Wen-hong* |
Central Laboratories, Medical College of Shantou University, Shantou 515041, China |
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Abstract A simple and reliable method was developed for the determination of serum copper and zinc in different chemical forms by graphite furnace atomic absorption spectrometry (GFAAS) with ethanol-EDTA precipitation. The serum and ethanol were mixed with volume ratio 1∶2. The mixture was incubated at 70 ℃ and ultra-centrifuged to precipitate proteins. Zinc and copper can be released from albumin after EDTA treating. Thus, macroglobulin-zinc, ceruloplasmin-copper, and albumin-bound zinc or copper could be determined by two proposed precipitation steps. The determination limit of copper (3σ) was 1.2 μg·L-1 and the recovery was 92.3%-104%, while the determination limit of zinc (3σ) was 0.098 μg·L-1, and the recovery was 90%-107%. This two-step precipitation method can be used to determine serum zinc and copper in various chemical forms in tumor, Wilson patients and heathy human.
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Received: 2009-09-02
Accepted: 2009-12-06
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Corresponding Authors:
LUO Wen-hong
E-mail: whluo@stu.edu.cn
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