| 光谱学与光谱分析 |
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| Determination of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in the Traditional Chinese Preparation by High Performance Liquid Chromatography with Chemiluminescence Detection |
| WEI Hong-qing1，2, LIU Er-bao1*, REN Rong-fang1, ZHAO Xiu-li2，LI Xiao-xia2, JIANG Feng-xian2 |
1. School of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, China
2. College of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004，China |
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Abstract Based on the chemiluminescence reaction of KMnO4-HNO3-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside sensitized by HCHO, an analytical method for 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside by RP-HPLC with chemiluminescence detector was developed. The chromatographic conditions were optimized. AN ODS hypersil column(25 mm×4.0 mm, 125×4.0 mm i.d. 5 μm) was used with acetonitrile-0.3% H3PO4[25∶75, (φ)] as mobile phase, and the flow rate was 0.8 mL·min-1 at the column temperature of 40 ℃. The linear range of calibration curve was from 15.75 to 136.5 μg·mL-1, the detection limit was 11.83 μg·mL-1 with a concentration of 21 μg·mL-1 of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, and the recovery was 102%-108%. The relative standard deviation was 3.45%(Cs=21.0 μg·mL-1, n=3). The method has been applied to the determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in a few Chinese medicines, and the results were satisfactory. The method was simple, rapid and highly selective.
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Received: 2003-12-16
Accepted: 2004-03-28
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Corresponding Authors:
LIU Er-bao
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| Cite this article: |
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WEI Hong-qing,LIU Er-bao,REN Rong-fang, et al. Determination of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in the Traditional Chinese Preparation by High Performance Liquid Chromatography with Chemiluminescence Detection [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 844-847.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I06/844 |
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