光谱学与光谱分析 |
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Study on the Malignant and Normal Rectum Tissues Using NMR Spectroscopy |
GAO Xiu-xiang1, HE Wen-yi2, YAO Hong-wei3, DU Jun-kai4, ZHAO Mei-xian1, 5, QI Jian1, LI Hui-zhen1, 6, XU Yi-zhuang1*, WU Jin-guang1 |
1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 2. Chinese Academy of Medical Sciences Peking Union College of Medicine, Institute of Materia Medica, Beijing 100050, China 3. The Third Hospital, Peking University, Beijing 100083, China 4. The First Hospital of Xi’an Jiaotong University, Xi’an 710061, China 5. Applied Chemistry Department, School of Science, Beijing University of Chemical Technology, Beijing 100029, China 6. College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China |
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Abstract In the present paper, NMR spectroscopy, an effective tool to detect the variation in molecular structure and changes in chemical composition of metabolites in tissues, was used to study the differences between malignant and normal tissues from rectum. 1H spectra of four malignant rectum tissue samples and two normal control tissues were investigated by using a 500M NMR high-resolution magic angle spinning magnetic resonance spectrometers (HR-MAS NMR). The results indicate that the 1H HR-MAS spectra of rectum cancer tissues are significantly different from those of the normal controls and most differences are presents in the form of variation in the relative intensities of the characteristic peak of various metabolites. In order to characterize the variation in the relative intensities in a quantitative manner, the intensity of the methyl peak of fatty acid at 0.88 was utilized as inner standard. Systematic differences between NMR spectra of malignant tissue and normal controls are as follows: (1) The concentration of amino acid increases significantly in malignant tissues, since the relative intensities of characteristic peaks of amino acid including valine, isoleucine, leucine, lysine, glutamate, glutamine, and aspartate are stronger in the NMR spectra of the malignant tissues. This phenomenon may reflect the fact that the activity of protein synthesis is enhanced in cancerous tissues. (2) The intensities of the characteristic peaks of lactic acid in malignant tissues are higher than those from normal controls. This may be related to the nature of anaerobic metabolism activity in malignant tissues. (3) The level of choline and its derivatives, taurine and creatine, increases significantly in malignant tissues, suggesting that the metabolic activity of malignant tissues changes. (4) In the spectral region between 4.5 and 10, observable changes occur on the peaks for unsaturated fatty acid and nuclear acids. Therefore, the above spectral variations in high resolution magic angle spinning NMR spectroscopy may be utilized as a potential tool to diagnose rectum cancer.
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Received: 2008-02-06
Accepted: 2008-04-26
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Corresponding Authors:
XU Yi-zhuang
E-mail: xiuxianggao@sina.com
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[1] WANG Fan,LING Xiao-feng,YANG Zhan-lan,et al(王 凡,凌晓峰,杨展澜,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(3):498. [2] XU Yi-zhuang,ZHAO Ying,XU Zhi,et al(徐怡庄,赵 莹,徐 智,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(11):1775. [3] YANG Li-min,XU Zhi,ZHANG Yuan-fu,et al(杨丽敏,徐 智,张元福,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(5):883. [4] PENG Qing, XU Yi-zhuang, LI Wei-hong, et al(彭 卿,徐怡庄,李维红,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),1998,18(5):528. [5] LI Wei-xiu,ZHENG Quan-qing,WANG Ping,et al(李炜修,郑全庆,王 平,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(10):1833. [6] ZHAO Yuan-li,L Jing,GE Xiang-hong,et al(赵元黎,吕 晶,葛向红,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(7):1267. [7] ZHAO Mei-xian,GAO Xiu-xiang,QI Jian,et al(赵梅仙,高秀香,齐 剑,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(2):308. [8] MENG Tao,XU Duan-fu,ZHAO Ying,et al(孟 涛,徐端夫,赵 莹,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(10):2069. [9] MENG Tao,XU Duan-fu,XU Yi-zhuang, et al(孟 涛,徐端夫,徐怡庄,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(6):1156. [10] CHEN Wen-xue,DENG Feng,YUE Yong(陈文学,邓 风,岳 勇). Chinese Journal of Megnetic Resonance(波谱学杂志),2004,21(1): 127. [11] Carolyn E Mountford,Sinead Doran,Cynthia L Lean,et al. Chem. Rev.,2004,104: 3677. [12] Kevin Millis,Patrick Weybright,Natalee Campbell,et al. Magnetic Resonance in Medicine,1999,41:257. [13] Beather Sitter,Ursula Sonnewald,Manfred Spraul,et al. NMR in Biomedicine,2002,15:327. [14] Beathe Sitter,Steinar Lundgren,Tone F Bathen,et al. NMR in Biomedicine,2006,19:30. [15] Marrita M Mahon,Andreanna D Williams,Soutter W Patrick,et al. NMR in Biomedicine,2004,17:1. [16] Marrita M Mahon,Nandita M deSouza,Roberto Dina,et al. NMR in Biomedicine,2004,17:144. [17] Martinez-Bisbal M Carmen,Luis Marti-Bonmati,José Piquer,et al. NMR in Biomedicine,2004,17:191. [18] ZHANG Hong-ping,SUN Jun-mo,TIAN Zhi-xiong,et al(张红萍,孙骏谟,田志雄,等). Journal of Clinical Radiololgy(临床放射学杂志),2005,24(4): 291. |
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