| 光谱学与光谱分析 |
|
|
|
|
|
| The Spectrum Studies of Mechano-Optical Signal Transduction with Participation of LDH |
| JIANG Ben-guo,ZHANG Le,FAN Sheng-di |
| Dalian Nationalities University,Key Lab of Biotechnology and Bioresources Utilization,SEAC-ME,Dalian 116600,China |
|
|
|
|
Abstract The optical response from a mechanical stimulus signal oscillating system of NADH,DPIP and O2 with the participant of lactate dehyrogenase (LDH) was studied with UV/Vis Spectrometer in the present paper. The signal transduction efficiency of the system was largely improved by the catalysis of LDH,when DPIP.∶NADH was 1∶4.5 (mole ratio)without lactate,and the average period of system was shortened from 108 to 34 min,and to 29 min with lactate existing.It was presumed that the accelerating effect of LDH was mainly brought by the activation of NADH at its catalysis center,and in addition,brought by the supplement of NADH through the dehydrogenation of lactate. The results indicated that the catalysis of enzyme would be put up also when only one of the two substrates existed.
|
|
Received: 2006-10-19
Accepted: 2007-01-22
|
|
|
|
Corresponding Authors:
JIANG Ben-guo
E-mail: jbg@dlnu.edu.cn
|
|
[1] Butcher E C,Berg E L,Kunkel E J. Nat. Biotechnol.,2004,22: 1253.
[2] Jun-ichi Kikuchi,Katsuhiko Ariga,Yoshihiro Sasaki,et al. Journal of Molecular Catalysis B: Enzymatic,2001,11(4-6): 977.
[3] Jun-ichi Kikuchi,Katsuhiko Ariga,Yoshihiro Sasaki. Advances in Supramolecular Chemistry,2002,8: 131.
[4] Sada K,Shiomi N,Miyata M. J. Am. Chem. Soc.,1998,120: 10543.
[5] Jenny E A Ottosson,Bakul C Dave. Sensors and Actuators,2004,B97: 198.
[6] Tariq M Rana,Claude F. Mearest. Proc. Nail. Acad. Sci. USA,1991,88: 10578.
[7] Sebastián Cerdán,Tiago B. Rodrigues,Alejandra Sierra,et al. Neurochemistry International,2006,48(6-7): 523.
[8] Kentaro Fukuda,Yoshihiro Sasaki,Katsuhiko Ariga,et al. Journal of Molecular Catalysis B: Enzymatic,2001,11(4-6): 971.
[9] Tian Wen-Jie,Yoshihiro Sasaki,Fan Sheng-Di,et al. Supramolecular Chemistry,2005，17: 113.
[10] Koichi Tanaka,Masaaki Honda,Toshikazu Takabatake. Journal of the American College of Cardiology,2001,37(2): 676.
[11] Yeong-Gon Choi,Eun-jin Bae,Dong-Seok Kim,et al. Journal of Dermatological Science,2006,43(3): 181.
[12] Roseata Zonouzi,Saeid Kazemi Ashtiani,Saman Hosseinkhani,et al. Journal of Biochemistry and Molecular Biology,2006,39(4): 426.
[13] JIANG Chun-ming,ZHANG Han-chang,LIN Xiang-qin,et al(姜春明,张汉昌,林祥钦,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,125(15): 751.
[14] James L Thomas,Ian Mason J,Stacey Brandt,et al. J. Biol. Chem.,2002,277(45): 42795.
[15] Murray J D. Math. Biol.,1993,19: 116. |
| [1] |
HUANG Bin, DU Gong-zhi, HOU Hua-yi*, HUANG Wen-juan, CHEN Xiang-bai*. Raman Spectroscopy Study of Reduced Nicotinamide Adenine Dinucleotide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1679-1683. |
| [2] |
REN Wen-jun1,4, XU Zheng-hong2, ZHANG Zhen-xi1*, YANG Xu-dong3, LI Zheng1 . Research on the Autofluorescence Spectroscopy in Rats Doing Medium-Intensity Exercise[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(05): 1331-1335. |
| [3] |
LI Yong-sheng1,GAO Xiu-feng2*. A New Method for Chemical Analysis: Fluorescence Capillary Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1565-1569. |
| [4] |
SUN Yun-xu, ZHU Feng, MA Wan-yun*. Study on Multi-Photon Excited Fluorescence Combined with Capillary Electrophoresis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(04): 502-505. |
|
|
|
|
