| 光谱学与光谱分析 |
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| Preparation of GSH Capped CdSe/CdS Core-Shell QDs and Labeling of Human T-Lymphocyte |
| DONG Wei1, 2, GE Xin2, WANG Xuan-yi2, XU Shu-kun1* |
1.Department of Chemistry, Northeastern University, Shenyang 110004, China
2.Department of Chemistry, Shenyang Medical College, Shenyang 110034, China |
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Abstract Two kinds of L-glutathione capped highly fluorescent CdSe/CdS core-shell quantum dots (QDs) emitting green and orange fluorescence at 350 nm excitation were firstly prepared by an aqueous approach and used as fluorescent labels, to link mouse anti-human CD3 which was expressed on human T-lymphocyte.UV-Vis absorption and fluorescence emission spectra of the as-prepared CdSe/CdS core-shell QDs were studied.Compared with the CdSe QDs, a remarkable enhancement in the emission intensity and a red shift of emission wavelength of CdSe/CdS core-shell QDs was observed for the two kinds of QDs emitting green and orange fluorescence.The TEM results showed that the as prepared CdSe and CdSe/CdS QDs dispersed well in aqueous solution, and their shape was approximately spherical, and the CdSe/CdS QDs nano particles emitting green fluorescence are of about 5 nm in diameter.The two kinds of CdSe/CdS QDs were linked with mouse anti-human CD3 to image human T-lymphocyte.The fluorescent microscopical images of human T-lymphocyte labeled with CdSe/CdS QDs-CD3 and FITC-CD3 demonstrated that the fluorescent CdSe/CdS QDs exhibited much better photo stability and brighter fluorescence than FITC, showing a good application potential in the immuno-labeling of cells.
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Received: 2009-01-29
Accepted: 2009-05-02
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Corresponding Authors:
XU Shu-kun
E-mail: xushukun46@1261.com
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[1] Michalet X, Pinaud F, Lacoste T D, et al.Single Molecules, 2001, 2(4): 261.
[2] Bruchez M, Moronne M, Gin P,et al.Science, 1998, 281: 2013.
[3] YANG Dong-zhi, XU Shu-kun, CHEN Qi-fan(杨冬芝，徐淑坤，陈启凡).Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2007, 27(9)： 1807.
[4] Gaponik N, Talapin D V, Rogach A L, et al.Phys.Chem.B, 2002, 106: 7177.
[5] Li H, Rothberg L.PNAS, 2004, 101: 14036.
[6] Spahnel L, Haase M, Weller H, et al.Journal of Am.Chem.Soc., 1987, 109: 5649.
[7] TANG Ai-wei, TENG Feng, GAO Yin-jie, et al(唐爱伟，滕 枫，高银洁，等).Journal of Inorganic Materials(无机材料学报)，2006，21(2)： 323.
[8] Yang D Z, Chen Q F, Xu S K.J.Luminescence, 2007; 126: 853.
[9] Jaiswal J K, Mattoussi H, Mauro J M, et al.Nature Biotechnol., 2003, 21: 47.
[10] Gao X, Nie S.Trends Biotechnol., 2003, 21: 371.
[11] Yang D Z, Chen Q F, Wang W X, et al.Luminescence, 2008, 23(3): 169.
[12] Chan W C W, Nie S M, Science, 1998, 281: 2016.
[13] Wu X Y, Liu H J, Liu J Q, et al.Nature Biotechnol., 2003, 21: 41.
[14] Wang H Z, Wang H Y, Liang R Q.Acta Bichimica et Biophyicas Sinica, 2004, 36(10): 681.
[15] Rajh T, Micc O I, Nozik AJ.J.Phy.Chem., 1993, 97: 11999.
[16] Zhu C Q, Zhao D H, Chen J L, et al.Anal.Bioanal.Chem., 2004, 378: 811.
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