| 光谱学与光谱分析 |
|
|
|
|
|
| Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method |
| HU Min1,2, ZHANG Zhen-xi1*,SHEN Guo-li3, LIU Ya-li3 |
1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, Xi’an Jiaotong University, Xi’an 710049, China
2. Department of Applied Chemistry, School of Science, Xi’an Jiaotong University, Xi′an 710049, China
3. State Key Laboratory for Chemo/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China |
|
|
|
|
Abstract The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 ×106 mol·L-1, and the binding site number is about 0.67 base pairs.
|
|
Received: 2005-06-16
Accepted: 2005-09-18
|
|
|
|
Corresponding Authors:
ZHANG Zhen-xi
|
|
| Cite this article: |
|
HU Min,ZHANG Zhen-xi,SHEN Guo-li, et al. Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1668-1671.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2006/V26/I09/1668 |
[1] LIU Liu, ZHANG Hai-tao, WANG Yun-pu(刘 流 ，张海涛，王云普). Chinese Journal of Organic Chemistry(有机化学), 2003, 23(6): 570.
[2] JIN Lan，YANG Pin(靳 兰, 杨 频). Chemical Journal of Chinese Universities(高等学校化学学报)，1996，17：1140.
[3] YAN Xun-ling, DONG Rui-xin, WANG Qiu-guo, et al(闫循领, 董瑞新, 王秋国, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005，25(1)：58.
[4] YU Ying, WU Lin, TAN Li-xian(俞 英, 吴 霖, 谭丽贤). Chinese Journal of Analytucal Chemistry(分析化学), 2004, 32(5): 628.
[5] SHEN Jing-shan, SUN Dan-dan, FU Lian-chun, et al(沈景山, 孙丹丹, 付连春, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(2): 232.
[6] LI Wen-you, WU Hui-ling, HE Xi-wen, et al(李文友, 吴会灵, 何锡文, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24(10): 1787.
[7] CHENG Gui-fang, DING Min, ZHAO Jie, et al(程圭芳, 丁 敏, 赵 洁, 等). Acta Chimica Sinica(化学学报)，2004，62(14)：1299.
[8] HU Min, YANG Xin, JIN Wei-jun, et al(胡 敏, 杨 欣, 晋卫军, 等). Acta Pharmaceutica Sinica(药学学报), 1999, 34(8): 608.
[9] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍，黄贤智，郑朱梓，等). Fluorescene Analyical Method(荧光分析法). Beijing: Science Press(北京：科学出版社), 1990.
[10] CAO Ying, LI Yi-jun, GAO Zhi, et al(曹 瑛, 李一峻, 高 志, 等). Acta Pharmaceutica Sinica(药学学报), 1999, 34(4)∶ 276.
[11] Kumar C V, Asuncion E H. J. Am. Chem. Soc., 1993, 115: 8547.
[12] McGhee J D, Ron Hippel P H. J. Mol. Biol., 1974, 86: 469.
[13] LI Yong，LI Yuan-zong, CHANG Wen-bao(李 勇, 李元宗, 常文保). Journal of Analytical Science(分析科学学报)，1994，10：67. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
YE Zi-yi, LIU Shuang, ZHANG Xin-feng*. Screening of DNA Dyes for Colorimetric Sensing Via Rapidly Inducing Gold Nanoparticles Aggregation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2805-2810. |
| [12] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [13] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [14] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [15] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
|
|
|
|
