| 光谱学与光谱分析 |
|
|
|
|
|
| Laser Tweezers Raman Spectroscopy Analysis of Liver Cancer Tissue |
| WANG Yan-jun1, YAO Hui-lu2, WANG Gui-wen2, WANG Yun1, FENG Mei-fu1* |
1. State Key Lab of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2. Laboratory for Biophysics, Guangxi Academy of Sciences, Nanning 530003, China |
|
|
|
|
Abstract Single cell laser tweezers Raman spectroscopy (LTRS) has been applied to biology field. In the present article, the authors measured the spectra of liver cancer cells, para-cancer cells and normal hepatocytes using single cell laser tweezer Raman spectroscopy (LTRS) system and compared their average spectra changes. The results showed that the laser tweezers Raman spectroscopy could differentiate specimens of different pathological changes from liver tissue studied. The 1 070 and 1 266 cm-1 peaks obtained from normal hepatocytes were more visible than the same two peaks obtained from liver cancer and para-cancer specimen. The 1 445 cm-1 peak of normal hepatocytes was higher than that of liver cancer cells and para-cancer cells. It is known that the 1 070 cm-1 peak represents lipids and nucleic acids, while 1 266 and 1 445 cm-1 peaks represent lipids and proteins. So, these peak changes may directly reflect the changed biomaterials related to liver carcinogenesis. Thus, single cell laser tweezer Raman spectroscopy may be a nondestructive, rapid and good method to measure and analyze different pathological specimens from liver cancer.
|
|
Received: 2007-09-02
Accepted: 2007-12-05
|
|
|
|
Corresponding Authors:
FENG Mei-fu
E-mail: fengmf@ioz.ac.cn
|
|
[1] Villanueva A, Newell P, Chiang D Y, et al. Seminarin Liver Disease, 2007, 27(1): 55.
[2] Hotta N, Ayada M, Okumura A, et al. Clin. Imaging., 2007, 31(2): 87.
[3] Sherley J L. Stem Cells, 2002, 20(6): 561.
[4] Lechler T, Fuchs E. Nature, 2005, 437(7056): 275.
[5] Potten C S, Loeffler M. Development, 1990, 110(4):1001.
[6] Stone N, Kendall C, Smith J, et al. Faraday Discussion, 2004, 126: 141, 169.
[7] Xie C A, Li Y Q. J. Appl. Physics, 2003, 93: 2982.
[8] Xie C A, Li Y Q, Tang W. J. Appl. Physics, 2003, 94: 6138.
[9] Tward A D, Jones K D, Yant S, et al. Proc. Natl. Acad. Sci. USA, 2007, 104(37): 14771.
[10] Nagahara T, Okano J, Murawaki Y. Oncol Rep., 2007, 18(5): 1281.
[11] Trencsényi G, Kertai P, Somogyi C, et al. DNA Cell Biol., 2007, 26(9): 649.
[12] Moriishi K, Mochizuki R. Proc. Natl. Acad. Sci. USA, 2007, 104(5): 1661.
[13] Yu Bo, Li Shiyong, An Ping, et al. World J. Gastroenterology, 2004, 10(18): 2652.
[14] LI Shi-yong, YU Bo, AN Ping, et al(李世拥, 于波, 安萍, 等). Chinese Journal of Surgery(中华外科杂志), 2004, 42(2): 84.
[15] FENG De-yun, ZHENG Hui, SHEN Ming, et al(冯德云, 郑晖, 沈明, 等). Journal of Central South University(Medical Science)(中南大学学报·医学版)，1999，24(4): 325.
|
| [1] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
| [2] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
| [3] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
| [4] |
WANG Xin-qiang1, 3, CHU Pei-zhu1, 3, XIONG Wei2, 4, YE Song1, 3, GAN Yong-ying1, 3, ZHANG Wen-tao1, 3, LI Shu1, 3, WANG Fang-yuan1, 3*. Study on Monomer Simulation of Cellulose Raman Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 164-168. |
| [5] |
WANG Lan-hua1, 2, CHEN Yi-lin1*, FU Xue-hai1, JIAN Kuo3, YANG Tian-yu1, 2, ZHANG Bo1, 4, HONG Yong1, WANG Wen-feng1. Comparative Study on Maceral Composition and Raman Spectroscopy of Jet From Fushun City, Liaoning Province and Jimsar County, Xinjiang Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 292-300. |
| [6] |
LI Wei1, TAN Feng2*, ZHANG Wei1, GAO Lu-si3, LI Jin-shan4. Application of Improved Random Frog Algorithm in Fast Identification of Soybean Varieties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3763-3769. |
| [7] |
WANG Zhi-qiang1, CHENG Yan-xin1, ZHANG Rui-ting1, MA Lin1, GAO Peng1, LIN Ke1, 2*. Rapid Detection and Analysis of Chinese Liquor Quality by Raman
Spectroscopy Combined With Fluorescence Background[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3770-3774. |
| [8] |
LIU Hao-dong1, 2, JIANG Xi-quan1, 2, NIU Hao1, 2, LIU Yu-bo1, LI Hui2, LIU Yuan2, Wei Zhang2, LI Lu-yan1, CHEN Ting1，ZHAO Yan-jie1*，NI Jia-sheng2*. Quantitative Analysis of Ethanol Based on Laser Raman Spectroscopy Normalization Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3820-3825. |
| [9] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
| [10] |
LI Qi-chen1, 2, LI Min-zan1, 2*, YANG Wei2, 3, SUN Hong2, 3, ZHANG Yao1, 3. Quantitative Analysis of Water-Soluble Phosphorous Based on Raman
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3871-3876. |
| [11] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
| [12] |
ZHU Hua-dong1, 2, 3, ZHANG Si-qi1, 2, 3, TANG Chun-jie1, 2, 3. Research and Application of On-Line Analysis of CO2 and H2S in Natural Gas Feed Gas by Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3551-3558. |
| [13] |
LIU Jia-ru1, SHEN Gui-yun2, HE Jian-bin2, GUO Hong1*. Research on Materials and Technology of Pingyuan Princess Tomb of Liao Dynasty[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3469-3474. |
| [14] |
LI Wen-wen1, 2, LONG Chang-jiang1, 2, 4*, LI Shan-jun1, 2, 3, 4, CHEN Hong1, 2, 4. Detection of Mixed Pesticide Residues of Prochloraz and Imazalil in
Citrus Epidermis by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3052-3058. |
| [15] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
|
|
|
|
