光谱学与光谱分析 |
|
|
|
|
|
Raman Spectra Study on Radiation Damage in EC9706 Cells by 60Coγ-Rays |
QI Jian,GUO Zheng-yuan,ZHANG Guang-shui,WU Dian-yong,TANG Wei-yue* |
College of Physics and Engineering, Zhengzhou University, Zhengzhou 450052,China |
|
|
Abstract Raman spectrum was used to study the structure and content of protein,nucleic acid and fat, while EC9706 cells irradiated by 60Co γ-ray were cultivated for 24 h. The results showed that for spectrum intensity and frequency deviation, there were big differences between each exposure group and control group. For the 1 244 cm-1 peak of amide Ⅲ, β folder changed to disordered conformations in the middle dose (4, 5Gy) groups. The 1 341 cm-1 peak of ν (the indole ring of Trp) was red-shifted in every dose group. There was a 2-3 cm-1 red shift at the 782 cm-1 peak in the big dose groups (7, 8Gy). It was showed that the non-hydrogenation of νs (PO-2) was strengthened due to big dose γ-rays radiation. There was a 4 cm-1 blue shift at the 1 446 cm-1 peak of δ (CH2, CH3). It maybe resulted from 60Co γ-rays’ damage to the film of EC9706 cells. The preferable dose of 60Co γ-rays may be found by analyzing the variety of the above-mentioned peaks in some dose groups.
|
Received: 2008-05-08
Accepted: 2008-08-12
|
|
Corresponding Authors:
TANG Wei-yue
E-mail: tangwy@zzu.edu.cn
|
|
[1] Morita A, Coffey R J, Foote R L, et al. Journal of Neurosurgery, 1999, 90: 42. [2] TANG Yi, LIU Wen-li, ZHOU Jian-feng, et al(汤屹, 刘文励, 周剑锋, 等). Chinese Journal of Radiological Medicine and Protection(中华放射医学与防护杂志 ), 2003, 23(6): 418. [3] Stock R G, Cahlon O, Cesaretti J A, et al. International Journal of Radiation Oncol. Biol. Phys., 2004, 59(5): 1352. [4] Ong G L, Elsamra S E, Goldenberg D M, et al. Clinical Cancer Res., 2001, 7(1): 192. [5] WANG Le-xin, ZHAO Zhi-min, YAO Hong-bing, et al(王乐新,赵志敏,姚红兵, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(6): 980. [6] Frank C J, McCreery R L, Redd D C B. Anal. Chem., 1995, 67: 777. [7] Redd D C B, Feng Z C, Yue K T, et al. Appl. Spectrosc., 1993, 47: 787. [8] Rigas B, Morgello S, Goldman I S, et al. Proc. Natl. Acad. Sci. USA, 1990, 87: 8140. [9] Liu C H, Das B B, Glassman W L S, et al. J. Photochem. Photobiol. B: Biol., 1992, 16: 187. [10] Hawi Sharon R, Campbell William B, Kajdacsy-Balla Andre, et al. Cancer Letters, 1996, 110: 35. [11] WU De-chang(吴德昌). Radiation Medicine(放射医学). Beijing: Military Medical Science Press(北京: 军事医学科学出版社), 2001. 29. [12] XU Yi-ming(许以明). Raman Spectrum in the Application of Structure Biology(拉曼光谱在结构生物学中的应用). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 13. [13] Barettino D, Vivanco Ruiz M, Stunnenberg H G. EMBO Journal, 1994, 13(13): 3039. [14] Gao Xiaoling, Butler Ian S, Kremer Richard. Spectrochimica Acta Part A. 2005, 61: 27.
|
[1] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
[2] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
[3] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
[4] |
WANG Xin-qiang1, 3, CHU Pei-zhu1, 3, XIONG Wei2, 4, YE Song1, 3, GAN Yong-ying1, 3, ZHANG Wen-tao1, 3, LI Shu1, 3, WANG Fang-yuan1, 3*. Study on Monomer Simulation of Cellulose Raman Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 164-168. |
[5] |
WANG Lan-hua1, 2, CHEN Yi-lin1*, FU Xue-hai1, JIAN Kuo3, YANG Tian-yu1, 2, ZHANG Bo1, 4, HONG Yong1, WANG Wen-feng1. Comparative Study on Maceral Composition and Raman Spectroscopy of Jet From Fushun City, Liaoning Province and Jimsar County, Xinjiang Province[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 292-300. |
[6] |
LI Wei1, TAN Feng2*, ZHANG Wei1, GAO Lu-si3, LI Jin-shan4. Application of Improved Random Frog Algorithm in Fast Identification of Soybean Varieties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3763-3769. |
[7] |
WANG Zhi-qiang1, CHENG Yan-xin1, ZHANG Rui-ting1, MA Lin1, GAO Peng1, LIN Ke1, 2*. Rapid Detection and Analysis of Chinese Liquor Quality by Raman
Spectroscopy Combined With Fluorescence Background[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3770-3774. |
[8] |
LIU Hao-dong1, 2, JIANG Xi-quan1, 2, NIU Hao1, 2, LIU Yu-bo1, LI Hui2, LIU Yuan2, Wei Zhang2, LI Lu-yan1, CHEN Ting1,ZHAO Yan-jie1*,NI Jia-sheng2*. Quantitative Analysis of Ethanol Based on Laser Raman Spectroscopy Normalization Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3820-3825. |
[9] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
[10] |
LI Qi-chen1, 2, LI Min-zan1, 2*, YANG Wei2, 3, SUN Hong2, 3, ZHANG Yao1, 3. Quantitative Analysis of Water-Soluble Phosphorous Based on Raman
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3871-3876. |
[11] |
GUO He-yuanxi1, LI Li-jun1*, FENG Jun1, 2*, LIN Xin1, LI Rui1. A SERS-Aptsensor for Detection of Chloramphenicol Based on DNA Hybridization Indicator and Silver Nanorod Array Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3445-3451. |
[12] |
ZHU Hua-dong1, 2, 3, ZHANG Si-qi1, 2, 3, TANG Chun-jie1, 2, 3. Research and Application of On-Line Analysis of CO2 and H2S in Natural Gas Feed Gas by Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3551-3558. |
[13] |
LIU Jia-ru1, SHEN Gui-yun2, HE Jian-bin2, GUO Hong1*. Research on Materials and Technology of Pingyuan Princess Tomb of Liao Dynasty[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3469-3474. |
[14] |
LI Wen-wen1, 2, LONG Chang-jiang1, 2, 4*, LI Shan-jun1, 2, 3, 4, CHEN Hong1, 2, 4. Detection of Mixed Pesticide Residues of Prochloraz and Imazalil in
Citrus Epidermis by Surface Enhanced Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3052-3058. |
[15] |
ZHAO Ling-yi1, 2, YANG Xi3, WEI Yi4, YANG Rui-qin1, 2*, ZHAO Qian4, ZHANG Hong-wen4, CAI Wei-ping4. SERS Detection and Efficient Identification of Heroin and Its Metabolites Based on Au/SiO2 Composite Nanosphere Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3150-3157. |
|
|
|
|