光谱学与光谱分析 |
|
|
|
|
|
Relationship between the Laccase Production of Pleurotus Ostreatus and the Full Wavelength Scan for the Fermentation Liquid |
CHENG Fan-sheng1, SHENG Ji-ping1, WANG Ren-ai1, SHEN Lin1, 2* |
1. College of Food Science and Nutrient Engineering, China Agricultural University, Beijing 100083, China 2. College of Food Science, Xinjiang Agricultural University, Urumqi 830052, China |
|
|
Abstract Laccase, widely distributed in fungi lacking high substrate specificity, plays an important role in lignin degradation in nature and environmental protection. In order to determine or estimate the laccase production during the fermentation of liquid media, the authors studied the full-length wave scan on the rough fermentation liquid of the Pleurotus ostreatus, which produces laccase high. Combined with the normal chemical method and diameter of the laccase and mycelium stain, which grew on the PDA (potato dextrose agar) plate with guaiacol added, we could get the exact information of laccase production. The result showed that the laccase activity increased in a rapid way in the first 5 days during the fermentation process, remained almost at the same level in the following 4 days, then increased rapidly until the 11 day, which was 148.7 U·L-1, increased 17.9 times. The diameter of laccase and mycelium stain increased with the culture time. The number of the wave peaks around 300 nm had a positive correlation with the laccase production; the peak width of OD over 1.5 around 300 nm had a positive correlation with the laccase production, which ranges from 5 nm on the first day to 80 nm on the 11th day. The light absorption line between the wavelengths 300 and 400 nm had a positive correlation with the laccase production with peaks at 349, 365 and 388 nm, and at 365 the peak gets its highest. Using these parameters, the authors could get the general production of the laccase production of liquid fermentation. Compared with the normal chemical method, the full-length wave scan method is much easier, cheap and simple. Furthermore, there are no special chemical substances used. It is really a new method for the evaluation and determination of laccase.
|
Received: 2008-06-26
Accepted: 2008-09-28
|
|
Corresponding Authors:
SHEN Lin
E-mail: pingshen@cau.edu.cn
|
|
[1] Alfred M, Mayera R. Phytochemistry, 2002, 60: 551. [2] Diego M, Angeles S. World Juornal of Micobiology and Biotechnology, 2006, 22(11): 1197. [3] Rebelo S, et al. Sensor., 2003, 3(6): 166. [4] ZHANG Min, XIAO Ya-zhong, GONG Wei-min(张 敏,肖亚中,龚为民). Journal of Biology(生物学杂志), 2003, 20(5): 6. [5] XU Ying, LAN Jin(许 颖,兰 进). Acta Edulis Fungi(食用菌学报), 2005, 12(1): 57. [6] Vianello F, Ragusas, Cambria M T, et al. Biosensors and Bioelectronocs, 2006, 21(11): 2155. [7] ZHU Si-ming, YU Shu-juan, YANG Lian-sheng(朱思明, 于淑娟, 杨连生). China Food Addictives(中国食品添加剂), 2005(11): 126. [8] WANG Yi-lei(王宜磊). Biotechnology(生物技术), 2003, 13(2): 9. [9] Mikuni J. FEMS Microbiology Letters., 1997, (155): 79. [10] WANG Jian-feng, WANG Zhang, RAO Jun, et al(王剑锋, 王 璋, 饶 军, 等). Mycosystema(菌物学报), 2008, 27(2): 297. [11] MAO Xiao-lan(卯晓岚). Chinese Economic Fungi(中国经济真菌). Beijing: Science Press(北京: 科学出版社), 1998. 33. [12] WU Juan, XIAO Ya-zhong, WANG Yi-ping, et al(吴 涓,肖亚中,王怡平,等). Journal of Xiamen University(厦门大学学报), 2001, 40(4): 843. [13] WANG Jian-feng, SU Guo-cheng, LIU Jian-ling(王剑锋,苏国成,刘建玲). Food and Fermentation Industry(食品与发酵工业), 2006, 32(2): 20. [14] WANG Yi-lei(王宜磊). Journal of Microbiology(微生物学杂志), 1998, 18(4): 60. [15] SHANG Yi, TAN Xiao-li, YUAN Hong-yu, et al(尚 毅,谭小力,袁红玉, 等). Acta Agriculturae Boreali-Occidentalis Sinica(西北农业学报), 2002, 11(1): 31. [16] JI Hai-yan(吉海彦). Analytical Instrumentation(分析仪器), 1998, (2): 30. [17] DONG Jia-li, HUANG Wei-zao, ZHANG Zheng-yi(董佳里, 黄维藻, 张正义). Journal of Sichuan University(Natural Science Edi.)(四川大学学报·自然科学版), 2004, 41(2): 431. [18] YANG Jian-ming, ZHANG Xiao-min, XING Zeng-tao(杨建明, 张小敏, 邢增涛). Mycosystema(菌物学报), 2005, 24(1): 61. [19] FU Ming-hui, KONG Min, HAN Ya-li(傅明辉,孔 敏,韩雅莉). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3): 532. [20] TU Chu-qiao, LIANG Hong, WANG Guang-hui(涂楚桥,梁 宏, 王光辉). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(4): 524.
|
[1] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
[2] |
YAN Ming-liang1, ZHANG Chen-long2, ZHAO Lian-xiang3, ZHAO Hua-he4, GAO Xun2*. Spectral Characteristics of Ge Plasma Induced by Femtosecond Pulsed Laser Ablation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2095-2098. |
[3] |
YUAN Kai-xin, ZHUO Jin, ZHANG Qing-hua, LI Ya-guo*. Study on the Spectral and Laser Damage Resistance of CO2 Laser Modified Sol-Gel SiO2 Thin Films[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1752-1759. |
[4] |
AN Huan1, YAN Hao-kui2, XIANG Mei1*, Bumaliya Abulimiti1*, ZHENG Jing-yan1. Spectral and Dissociation Characteristics of p-Dibromobenzene Based on External Electric Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 405-411. |
[5] |
CHEN Feng-nong1, SANG Jia-mao1, YAO Rui1, SUN Hong-wei1, ZHANG Yao1, ZHANG Jing-cheng1, HUANG Yun2, XU Jun-feng3. Rapid Nondestructive Detection and Spectral Characteristics Analysis of Factors Affecting the Quality of Dendrobium Officinale[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3276-3280. |
[6] |
WANG Wen, QIU Gui-hua*, PAN Shi-bing, ZHANG Rui-rong, HAN Jian-long, WANG Yi-ke, GUO Yu, YU Ming-xun. Terahertz Absorption and Molecular Vibration Characteristics of PA66 Polymer Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2702-2706. |
[7] |
WANG Yuan1, 2, 3, WANG Jin-liang1, 2, 3*. Chlorophyll Fluorescence-Spectral Characteristics of Vegetables Under Different Fertilizer Treatments[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2427-2433. |
[8] |
WU Qi-jun1, DU Qing1, HAN Li-min1, WANG Ling-xuan2. Study on Physical Properties and Spectra of AlO in External Radiation Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1023-1027. |
[9] |
FANG Zi-qiu1,2, CHEN Guo-qing1,2*, WU Ya-min1,2. Studyon the Spectral Properties of Riboflavin in Different Polar Solvents[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1132-1136. |
[10] |
LI Qing-ling1, 2, 3, YIN Da-yi1, 2, 3*. A Study of Spectral Polarization Properties of Oil Slick with Ellipsometry from Ultraviolet to Near-Infrared[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(06): 1661-1666. |
[11] |
WANG Jin-xia1, LUO Le1, CHEN Yu-cheng2, HE Qing-ming3, ZHAN Ling-ling1, ZHAO Xue1. Spectra Characteristic and Algicidal Mechanism Of Chryseobaterium sp. S7 on Microcystis Aeruginosa[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(06): 1817-1822. |
[12] |
ZHANG Zhi-heng, ZHAO Fei, YANG Wen*, MO Jing-hui, GE Wen, LI Xue-ming, YANG Pei-zhi. Effect of Pulse Power on the Phase Structure and Spectral Properties of SiCx Thin Films Containing Si Quantum Dots[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 529-534. |
[13] |
LI Wen-cui1, DONG Gang-song1, LIU Yan2, LIU Yong-gang2. Study on the Spectrum Characteristics of Dye Doped Liquid Crystal Laser[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3273-3277. |
[14] |
WANG Qiang1, LI Xin-yi1*, CHANG Tian-ying2, 3, HU Qiu-ping1, BAI Jin-peng4. Terahertz Time-Domain Spectroscopic Study of Aircraft Composite and Matrix Resins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2706-2712. |
[15] |
WU Fu-fei1, 2, DONG Shuang-kuai1*, ZHAO Zhen-hua1, GONG Jing-wei2, SHI Ke-bin2. Stuay of Mineral Admixtures as Fine Aggregate on Hydration Products and Spectral Performance of Mortar[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2853-2859. |
|
|
|
|